Editor: Michael Wagner
On the reproducibility of microcosm experiments – different community composition in parallel phototrophic biofilm microcosms
Version of Record online: 26 JUN 2006
FEMS Microbiology Ecology
Volume 58, Issue 2, pages 169–178, November 2006
How to Cite
Roeselers, G., Zippel, B., Staal, M., Van Loosdrecht, M. and Muyzer, G. (2006), On the reproducibility of microcosm experiments – different community composition in parallel phototrophic biofilm microcosms. FEMS Microbiology Ecology, 58: 169–178. doi: 10.1111/j.1574-6941.2006.00172.x
- Issue online: 26 JUN 2006
- Version of Record online: 26 JUN 2006
- Received 20 January 2006; revised 21 March 2006; accepted 24 March 2006.First published online 26 June 2006.
- microbial ecology;
- oxygenic phototrophs
Phototrophic biofilms were cultivated simultaneously using the same inoculum in three identical flow-lane microcosms located in different laboratories. The growth rates of the biofilms were similar in the different microcosms, but denaturing gradient gel electrophoresis (DGGE) analysis of both 16S and 18S rRNA gene fragments showed that the communities developed differently in terms of species richness and community composition. One microcosm was dominated by Microcoleus and Phormidium species, the second microcosm was dominated by Synechocystis and Phormidium species, and the third microcosm was dominated by Microcoleus- and Planktothrix-affiliated species. No clear effect of light intensity on the cyanobacterial community composition was observed. In addition, DGGE profiles obtained from the cultivated biofilms showed a low resemblance with the profiles derived from the inoculum. These findings demonstrate that validation of reproducibility is essential for the use of microcosm systems in microbial ecology studies.