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Figure S1. Eukaryal 18S rRNA gene denaturing gradient electrophoresis gel showing distribution of phylotypes at different depths in the ice cover. Total number of bands per sample (as determined by GelComparII and visually confirmed) listed at the bottom of each lane. Numbers to the left of individual bands indicate bands that were excised and sequenced. Arrow pointing to the only universal phylotype (note low intensity in some samples). Figure S2. Bacterial 16S rRNA gene denaturing gradient electrophoresis gel showing distribution of phylotypes at different depths in the ice cover. Total number of bands per sample (as determined by GelComparII and visually confirmed) listed at the bottom of each lane. Arrows pointing to universal phylotypes (note low intensity in some samples). Figure S3. 16S rRNA gene clone library richness and coverage estimates. Predicted SChao1 values (panel A) and predicted CACE values (panel B) for the Lake Vida 4.8 m (filled circles) and 15.9 m (open circles) clone libraries. Table S1. Eukaryal and bacterial DGGE profile pairwise similarities (Sorenson’s index).

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FEM+220_FigsS1-S3.pdf576KSupporting info item
FEM+220_Table+S1.pdf15KSupporting info item

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