*Universidad Nacional del Comahue-Consejo Nacional de Investigaciones Científicas y Tecnológicas
Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina
Article first published online: 15 JAN 2007
FEMS Microbiology Ecology
Volume 59, Issue 2, pages 331–341, February 2007
How to Cite
De García, V., Brizzio, S., Libkind, D., Buzzini, P. and Van Broock, M. (2007), Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina. FEMS Microbiology Ecology, 59: 331–341. doi: 10.1111/j.1574-6941.2006.00239.x
Editor: Rosa Margesin
- Issue published online: 15 JAN 2007
- Article first published online: 15 JAN 2007
- Received 29 April 2006; revised 20 August 2006; accepted 13 September 2006.First published online January 2007.
- glacial meltwater environment
The occurrence of culturable yeasts in glacial meltwater from the Frías, Castaño Overo and Río Manso glaciers, located on Mount Tronador in the Nahuel Huapi National Park (Northwestern Patagonia, Argentina) is presented. Subsurface water samples were filtered for colony counting and yeast isolation. The total yeast count ranged between 6 and 360 CFU L–1. Physiologic and molecular methods were employed to identify 86 yeast isolates. In agreement with yeast diversity data from studies for Antarctic and Alpine glaciers, the genera Cryptococcus, Leucosporidiella, Dioszegia, Rhodotorula, Rhodosporidium, Mrakia, Sporobolomyces, Udeniomyces and Candida were found. Cryptococcus and Leucosporidiella accounted for 50% and 20% of the total number of strains, respectively. Among 21 identified yeast species, Cryptococcus sp. 1 and Leucosporidiella fragaria were the most frequent. The typically psychrophilic Mrakia yeast strain and three new yeast species, yet to be described, were also isolated. All yeast strains were able to grow at 5, 10, and 15°C. Among yeast strains expressing extracellular enzymatic activity, higher proteolytic and lipolytic activities were obtained at 4°C than at 20°C.