Relationships between the Arctic and the Antarctic cyanobacteria; three Phormidium-like strains evaluated by a polyphasic approach

Authors

  • Katia Comte,

    1. Institute of Botany, Academy of Sciences of the Czech Republic, Třeboň and Faculty of Biological Sciences, University of South Bohemia, České Budějovice, Czech Republic
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  • Marie Šabacká,

    1. Institute of Botany, Academy of Sciences of the Czech Republic, Třeboň and Faculty of Biological Sciences, University of South Bohemia, České Budějovice, Czech Republic
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  • Alyssa Carré-Mlouka,

    1. Laboratoire de Génétique et Biologie Cellulaire, Université de Versailles-St Quentin en Yvelines, UFR des Sciences, Versailles, France
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  • Josef Elster,

    1. Institute of Botany, Academy of Sciences of the Czech Republic, Třeboň and Faculty of Biological Sciences, University of South Bohemia, České Budějovice, Czech Republic
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  • Jiří Komárek

    1. Institute of Botany, Academy of Sciences of the Czech Republic, Třeboň and Faculty of Biological Sciences, University of South Bohemia, České Budějovice, Czech Republic
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  • Present address: Katia Comte, School of Biological Sciences, University of Bristol, Bristol, UK.

  • Editor: Max Häggblom

Correspondence: Katia Comte, School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK. Tel.: +44 117 928 75 92; fax: +44 117 925 73 74; e-mail: katia.comte@bristol.ac.uk

Abstract

Selected strains of filamentous Phormidium-like cyanobacteria isolated from two Arctic regions (Ellesmere Island, High Canadian Arctic and Svalbard) and from Antarctica (Antarctic peninsula, South Shetland Islands and South Orkney Islands) were studied. The polyphasic approach used included phenotypic observations of morphological features and genotypic analyses (restriction fragment length polymorphism of 16S rRNA gene, internal transcribed space, 16S rRNA gene sequence analysis). Although genotypes generally correspond to observed morphotypes, the genetic analyses revealed a high degree of biodiversity that could not be unveiled using solely morphological evaluations. According to the phylogenetic analysis, the three clones were divided into two major clades, indicating that the phylogenetic distance between Arct-Ph5/Ant-Ph68 and Ant-Ph58 was so large they belonged to different genera. The polyphyletic position of strains of the genus Phormidium was confirmed by this study, attesting the need to entirely revise classification in this taxon in the future.

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