Editor: Max Häggblom
Motility and chemotaxis of Pseudomonas sp. B4 towards polychlorobiphenyls and chlorobenzoates
Article first published online: 20 MAR 2007
FEMS Microbiology Ecology
Volume 60, Issue 2, pages 322–328, May 2007
How to Cite
Gordillo, F., Chávez, F. P. and Jerez, C. A. (2007), Motility and chemotaxis of Pseudomonas sp. B4 towards polychlorobiphenyls and chlorobenzoates. FEMS Microbiology Ecology, 60: 322–328. doi: 10.1111/j.1574-6941.2007.00293.x
- Issue published online: 20 MAR 2007
- Article first published online: 20 MAR 2007
- Received 22 September 2006; revised 21 December 2006; accepted 22 December 2006.First published online 20 March 2007.
- environmental pollution
The polychlorinated biphenyl (PCB)-degrading Pseudomonas sp. B4 was tested for its motility and ability to sense and respond to biphenyl, its chloroderivatives and chlorobenzoates in chemotaxis assays. Pseudomonas sp. B4 was attracted to biphenyl, PCBs and benzoate in swarm plate and capillary assays. Chemotaxis towards these compounds correlated with their use as carbon and energy sources. No chemotactic effect was observed in the presence of 2- and 3-chlorobenzoates. Furthermore, a toxic effect was observed when the microorganism was exposed to 3-chlorobenzoate. A nonmotile Pseudomonas sp. B4 transformant and Burkholderia xenovorans LB400, the laboratory model strain for PCB degradation, were both capable of growing in biphenyl as the sole carbon source, but showed a clear disadvantage to access the pollutants to be degraded, compared with the highly motile Pseudomonas sp. B4, stressing the importance of motility and chemotaxis in this environmental biodegradation.