Editor: Julian Marchesi
Characterization of the xylan-degrading microbial community from human faeces
Version of Record online: 28 MAR 2007
FEMS Microbiology Ecology
Volume 61, Issue 1, pages 121–131, July 2007
How to Cite
Chassard, C., Goumy, V., Leclerc, M., Del'homme, C. and Bernalier-Donadille, A. (2007), Characterization of the xylan-degrading microbial community from human faeces. FEMS Microbiology Ecology, 61: 121–131. doi: 10.1111/j.1574-6941.2007.00314.x
- Issue online: 28 MAR 2007
- Version of Record online: 28 MAR 2007
- Received 27 October 2006; revised 2 February 2007; accepted 9 February 2007.First published online 28 March 2007.
- human colon;
- xylan degradation;
- xylanolytic bacteria;
- Bacteroides sp.;
- Roseburia sp
In humans, plant cell wall polysaccharides represent an important source of dietary fibres that are digested by gut microorganisms. Despite the extensive degradation of xylan in the colon, the population structure and the taxonomy of the predominant bacteria involved in degradation of this polysaccharide have not been extensively explored. The objective of our study was to characterize the xylanolytic microbial community from human faeces, using xylan from different botanic origins. The xylanolytic population was enumerated at high level in all faecal samples studied. The predominant xylanolytic organisms further isolated (20 strains) were assigned to Roseburia and Bacteroides species. Some Bacteroides isolates corresponded to the two newly described species Bacteroides intestinalis and Bacteroides dorei. Other isolates were closely related to Bacteroides sp. nov., a cellulolytic bacterium recently isolated from human faeces. The remaining Bacteroides strains could be considered to belong to a new species of this genus. Roseburia isolates could be assigned to the species Roseburia intestinalis. The xylanase activity of the Bacteroides and Roseburia isolates was found to be higher than that of other gut xylanolytic species previously identified. Our results provide new insights to the diversity and activity of the human gut xylanolytic community. Four new xylan-degrading Bacteroides species were identified and the xylanolytic capacity of R. intestinalis was further shown.