Editor: Gary King
An ice-binding protein from an Antarctic sea ice bacterium
Article first published online: 21 JUL 2007
FEMS Microbiology Ecology
Volume 61, Issue 2, pages 214–221, August 2007
How to Cite
Raymond, J. A., Fritsen, C. and Shen, K. (2007), An ice-binding protein from an Antarctic sea ice bacterium. FEMS Microbiology Ecology, 61: 214–221. doi: 10.1111/j.1574-6941.2007.00345.x
- Issue published online: 21 JUL 2007
- Article first published online: 21 JUL 2007
- Received 28 November 2006; revised 16 April 2007; accepted 24 April 2007.First published online July 2007.
- sea ice;
- ice-binding protein;
An Antarctic sea ice bacterium of the Gram-negative genus Colwellia, strain SLW05, produces an extracellular substance that changes the morphology of growing ice. The active substance was identified as a ∼25-kDa protein that was purified through its affinity for ice. The full gene sequence was determined and was found to encode a 253-amino acid protein with a calculated molecular mass of 26 350 Da. The predicted amino acid sequence is similar to predicted sequences of ice-binding proteins recently found in two species of sea ice diatoms and a species of snow mold. A recombinant ice-binding protein showed ice-binding activity and ice recrystallization inhibition activity. The protein is much smaller than bacterial ice-nucleating proteins and antifreeze proteins that have been previously described. The function of the protein is unknown but it may act as an ice recrystallization inhibitor to protect membranes in the frozen state.