Present address: Bärbel U. Foesel, Bereich Mikrobiologie, Department Biologie I Ludwig-Maximilians-Universität München, Germany. Eddie Cytryn, Department of Soil Water and Climate and Biotechnology Institute, University of Minnesota, Saint Paul, MN, USA.
Nitrosomonas Nm143-like ammonia oxidizers and Nitrospira marina-like nitrite oxidizers dominate the nitrifier community in a marine aquaculture biofilm
Article first published online: 17 DEC 2007
FEMS Microbiology Ecology
Volume 63, Issue 2, pages 192–204, February 2008
How to Cite
Foesel, B. U., Gieseke, A., Schwermer, C., Stief, P., Koch, L., Cytryn, E., De La Torré, J. R., Van Rijn, J., Minz, D., Drake, H. L. and Schramm, A. (2008), Nitrosomonas Nm143-like ammonia oxidizers and Nitrospira marina-like nitrite oxidizers dominate the nitrifier community in a marine aquaculture biofilm. FEMS Microbiology Ecology, 63: 192–204. doi: 10.1111/j.1574-6941.2007.00418.x
Editor: Michael Wagner
- Issue published online: 17 DEC 2007
- Article first published online: 17 DEC 2007
- Received 26 June 2007; revised 16 October 2007; accepted 21 October 2007.First published online 17 December 2007.
- marine aquaculture;
- nitrifying biofilm;
- DNA extraction;
- Nitrosomonas sp. Nm143;
- Nitrospira marina
Zero-discharge marine aquaculture systems are an environmentally friendly alternative to conventional aquaculture. In these systems, water is purified and recycled via microbial biofilters. Here, quantitative data on nitrifier community structure of a trickling filter biofilm associated with a recirculating marine aquaculture system are presented. Repeated rounds of the full-cycle rRNA approach were necessary to optimize DNA extraction and the probe set for FISH to obtain a reliable and comprehensive picture of the ammonia-oxidizing community. Analysis of the ammonia monooxygenase gene (amoA) confirmed the results. The most abundant ammonia-oxidizing bacteria (AOB) were members of the Nitrosomonas sp. Nm143-lineage (6.7% of the bacterial biovolume), followed by Nitrosomonas marina-like AOB (2.2% of the bacterial biovolume). Both were outnumbered by nitrite-oxidizing bacteria of the Nitrospira marina-lineage (15.7% of the bacterial biovolume). Although more than eight other nitrifying populations were detected, including Crenarchaeota closely related to the ammonia-oxidizer ‘Nitrosopumilus maritimus’, their collective abundance was below 1% of the total biofilm volume; their contribution to nitrification in the biofilter is therefore likely to be negligible.