Editor: Gary King
Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities
Article first published online: 3 MAR 2008
© 2008 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd
FEMS Microbiology Ecology
Volume 64, Issue 2, pages 240–247, May 2008
How to Cite
Nunoura, T., Oida, H., Miyazaki, J., Miyashita, A., Imachi, H. and Takai, K. (2008), Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities. FEMS Microbiology Ecology, 64: 240–247. doi: 10.1111/j.1574-6941.2008.00451.x
- Issue published online: 3 MAR 2008
- Article first published online: 3 MAR 2008
- Received 8 August 2007; revised 25 October 2007; accepted 29 December 2007.First published online 3 March 2008.
- anaerobic methanotroph;
- anaerobic oxidation of methane;
- quantitative fluorescent PCR
A quantitative fluorogenic PCR method for detecting methanogenic and methanotrophic orders was established using a refined primer set for the methyl coenzyme M reductase subunit A gene (mcrA). The method developed was applied to several microbial communities in which diversity and abundance of methanogens or anaerobic methanotrophs (ANMEs) was identified by 16S rRNA gene clone analysis, and strong correlations between the copy numbers of mcrA with those of archaeal 16S rRNA genes in the communities were observed. The assay can be applied to detecting and assessing the abundance of methanogens and/or ANMEs in anoxic environments that could not be detected by 16S rRNA gene sequence analyses.