Present address: Xosé Anxelu G. Morán, The Ecosystems Center, MBL, 7 MBL Street, Woods Hole, MA 02543-1015, USA.
Single-cell vs. bulk activity properties of coastal bacterioplankton over an annual cycle in a temperate ecosystem
Article first published online: 13 OCT 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 67, Issue 1, pages 43–56, January 2009
How to Cite
Morán, X. A. G. and Calvo-Díaz, A. (2009), Single-cell vs. bulk activity properties of coastal bacterioplankton over an annual cycle in a temperate ecosystem. FEMS Microbiology Ecology, 67: 43–56. doi: 10.1111/j.1574-6941.2008.00601.x
Editor: Patricia Sobecky
- Issue published online: 9 DEC 2008
- Article first published online: 13 OCT 2008
- Received 11 April 2008; revised 12 August 2008; accepted 20 August 2008.First published online 13 October 2008.
- single-cell properties;
- bacterial activity;
- seasonal variations;
The connections between single-cell activity properties of heterotrophic planktonic bacteria and whole community metabolism are still poorly understood. Here, we show flow cytometry single-cell analysis of membrane-intact (live), high nucleic acid (HNA) content and actively respiring (CTC+) bacteria with samples collected monthly during 2006 in northern Spain coastal waters. Bulk activity was assessed by measuring 3H-Leucine incorporation and specific growth rates. Consistently, different single-cell relative abundances were found, with 60–100% for live, 30–84% for HNA and 0.2–12% for CTC+ cells. Leucine incorporation rates (2–153 pmol L−1 h−1), specific growth rates (0.01–0.29 day−1) and the total and relative abundances of the three single-cell groups showed marked seasonal patterns. Distinct depth distributions during summer stratification and different relations with temperature, chlorophyll and bacterial biovolume suggest the existence of different controlling factors on each single-cell property. Pooled leucine incorporation rates were similarly correlated with the abundance of all physiological groups, while specific growth rates were only substantially explained by the percentage of CTC+ cells. However, the ability to reduce CTC proved notably better than the other two single-cell properties at predicting bacterial bulk rates within seasons, suggesting a tight linkage between bacterial individual respiration and biomass production at the community level.