Present address: Gwenaëlle Le Blay, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESMISAB, Université Européenne de Bretagne – Université de Brest, Technopôle Brest-Iroise, 29280 Plouzané, France.
New in vitro colonic fermentation model for Salmonella infection in the child gut
Article first published online: 12 DEC 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 67, Issue 2, pages 198–207, February 2009
How to Cite
Le Blay, G., Rytka, J., Zihler, A. and Lacroix, C. (2009), New in vitro colonic fermentation model for Salmonella infection in the child gut. FEMS Microbiology Ecology, 67: 198–207. doi: 10.1111/j.1574-6941.2008.00625.x
Editor: Julian Marchesi
- Issue published online: 22 DEC 2008
- Article first published online: 12 DEC 2008
- Received 11 June 2008; revised 4 September 2008; accepted 10 October 2008.First published online 12 December 2008.
- immobilized cells;
- intestinal microbiota;
- in vitro continuous fermentation model;
- Salmonella serovar Typhimurium
In this study, a new in vitro continuous colonic fermentation model of Salmonella infection with immobilized child fecal microbiota and Salmonella serovar Typhimurium was developed for the proximal colon. This model was then used to test the effects of two amoxicillin concentrations (90 and 180 mg day−1) on the microbial composition and metabolism of the gut microbiota and on Salmonella serovar Typhimurium during a 43-day fermentation. Addition of gel beads (2%, v/v) colonized with Salmonella serovar Typhimurium in the reactor resulted in a high and stable Salmonella concentration (log 7.5 cell number mL−1) in effluent samples, and a concomitant increase of Enterobacteriaeceae, Clostridium coccoides–Eubacterium rectale and Atopobium populations and a decrease of bifidobacteria. During amoxicillin treatments, Salmonella concentrations decreased while microbial balance and activity were modified in agreement with in vivo data, with a marked decrease in C. coccoides–E. rectale and an increase in Enterobacteriaceae. After interruption of antibiotic addition, Salmonella concentration again increased to reach values comparable to that measured before antibiotic treatments, showing that our model can be used to simulate Salmonella shedding in children as observed in vivo. This in vitro model could be a useful tool for developing and testing new antimicrobials against enteropathogens.