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Fig. S1. TRFLP profiles from different vents and time points throughout the course of this study.

Fig. S2. Thermo/chemical trends. Fluid temperature and chemistry plots from each vent over the course of the time series. Vents are abbreviated as follows: Marshmallow (MA), Gollum (GO), Bag City (BC), Marker 33 (33) and Cloud (CL). A) Maximum observed temperature, B) H2S/Si (mol/mol) C)H2/heat (nmol/J) D) Fe/heat (nmol/J).

Fig. S3. Primer 2D similarity MDS plots comparing vent samples via (a) dilution-dependent variables: Tmax, [Mg], [H2S], [Fe], and pH, (b) reaction-dependent variables: CH4/heat, H2S/heat, Fe/Mn and Fe/heat, and (c) all 16 thermo/chemical parameters. Vent time points closer together have similar environmental conditions (as measured by thermo/chemical parameters). Vents are abbreviated as follows: Marshmallow (MA), Gollum (GO), Bag City (BC), Marker 33 (33) and Cloud (CL).

Table S1. Fluid temperature and chemical parameters measured and used as part of this study.

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FilenameFormatSizeDescription
FEM_747_sm_FigS1.pdf171KSupporting info item
FEM_747_sm_FigS2.pdf127KSupporting info item
FEM_747_sm_FigS3.pdf71KSupporting info item
FEM_747_sm_TableS1.pdf274KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.