Editor: Philippe Lemanceau
Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatum truffle-ground
Article first published online: 9 SEP 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 71, Issue 1, pages 43–49, January 2010
How to Cite
Zampieri, E., Murat, C., Cagnasso, M., Bonfante, P. and Mello, A. (2010), Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatum truffle-ground. FEMS Microbiology Ecology, 71: 43–49. doi: 10.1111/j.1574-6941.2009.00783.x
- Issue published online: 10 DEC 2009
- Article first published online: 9 SEP 2009
- Received 28 May 2009; revised 24 July 2009; accepted 24 August 2009.Final version published online 22 September 2009.
- Tuber magnatum;
- nested PCR;
- specific primers
Truffles are hypogeous ectomycorrhizal fungi. They belong to the genus Tuber and are currently considered a hot spot in fungal biology due to their ecological and economic relevance. Among all the species, Tuber magnatum is the most appreciated because of its special taste and aroma. The aim of this work was to set up a protocol to detect T. magnatum in soil and to assess its distribution in a natural truffle-ground. We used the β-tubulin gene as a marker to identify T. magnatum in the soil. This gene allowed us to trace the distribution of the fungus over the entire truffle-ground. Tuber magnatum was found, in one case, 100 m from the productive host plant. This study highlights that T. magnatum mycelium is more widespread than can be inferred from the distribution of truffles and ectomycorrhizas. Interestingly, a new haplotype – never described from fruiting body material – was identified. The specific detection of T. magnatum in the soil will allow to unravel the ecology of this fungus, following its mycelial network. Moreover, this new tool may have practical importance in projects aimed to increase large-scale truffle production, checking for T. magnatum persistence in plantations.