Editor: Max Häggblom
Enrichment and characterization of a sulfate-reducing toluene-degrading microbial consortium by combining in situ microcosms and stable isotope probing techniques
Article first published online: 3 NOV 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 71, Issue 2, pages 237–246, February 2010
How to Cite
Bombach, P., Chatzinotas, A., Neu, T. R., Kästner, M., Lueders, T. and Vogt, C. (2010), Enrichment and characterization of a sulfate-reducing toluene-degrading microbial consortium by combining in situ microcosms and stable isotope probing techniques. FEMS Microbiology Ecology, 71: 237–246. doi: 10.1111/j.1574-6941.2009.00809.x
- Issue published online: 24 DEC 2009
- Article first published online: 3 NOV 2009
- Received 19 June 2009; revised 26 October 2009; accepted 26 October 2009.Final version published online 27 November 2009.
- stable isotope probing (SIP);
- in situ microcosm;
A toluene-degrading microbial consortium was enriched directly in a BTEX-contaminated aquifer under sulfate-reducing conditions using in situ microcosms consisting of toluene-loaded activated carbon pellets. Degradation of toluene and concomitant sulfide production by the consortium was subsequently demonstrated in laboratory microcosms. The consortium was physiologically and phylogenetically characterized by isotope tracer experiments using nonlabeled toluene, [13C]-α-toluene or [13C7]-toluene as growth substrates. Cells incubated with [13C]-α-toluene or [13C7]-toluene incorporated 8–15 at.%13C and 51–57 at.%13C into total lipid fatty acids, respectively, indicating a lower specific incorporation of 13C from [13C7]-toluene. In order to identify the toluene-assimilating bacteria, the incorporation of carbon from both [13C]-α-toluene and [13C7]-toluene into rRNA was analyzed by stable isotope probing. Time and buoyant density-resolved 16S rRNA gene-based terminal restriction fragment length polymorphism profiles, combined with cloning and sequencing, revealed that an uncultured bacterium (99% sequence similarity) related to the genus Desulfocapsa was the main toluene-degrading organism in the consortium. The ratio of the respective terminal restriction fragments changed over time, indicating trophic interactions within this consortium.