Editor: Max Häggblom
Expression of acetate permease-like (apl ) genes in subsurface communities of Geobacter species under fluctuating acetate concentrations
Article first published online: 10 MAY 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 73, Issue 3, pages 441–449, September 2010
How to Cite
Elifantz, H., N'Guessan, L. A., Mouser, P. J., Williams, K. H., Wilkins, M. J., Risso, C., Holmes, D. E., Long, P. E. and Lovley, D. R. (2010), Expression of acetate permease-like (apl ) genes in subsurface communities of Geobacter species under fluctuating acetate concentrations. FEMS Microbiology Ecology, 73: 441–449. doi: 10.1111/j.1574-6941.2010.00907.x
- Issue published online: 3 AUG 2010
- Article first published online: 10 MAY 2010
- Received 23 January 2010; revised 27 April 2010; accepted 27 April 2010.Final version published online 1 June 2010.
- apl genes;
The addition of acetate to uranium-contaminated aquifers in order to stimulate the growth and activity of Geobacter species that reduce uranium is a promising in situ bioremediation option. Optimizing this bioremediation strategy requires that sufficient acetate be added to promote Geobacter species growth. We hypothesized that under acetate-limiting conditions, subsurface Geobacter species would increase the expression of either putative acetate symporters genes (aplI and aplII). Acetate was added to a uranium-contaminated aquifer (Rifle, CO) in two continuous amendments separated by 5 days of groundwater flush to create changing acetate concentrations. While the expression of aplI in monitoring well D04 (high acetate) weakly correlated with the acetate concentration over time, the transcript levels for this gene were relatively constant in well D08 (low acetate). At the lowest acetate concentrations during the groundwater flush, the transcript levels of aplII were the highest. The expression of aplII decreased 2–10-fold upon acetate reintroduction. However, the overall instability of acetate concentrations throughout the experiment could not support a robust conclusion regarding the role of apl genes in response to acetate limitation under field conditions, in contrast to previous chemostat studies, suggesting that the function of a microbial community cannot be inferred based on lab experiments alone.