Editor: Julian Marchesi
Temporal shifts of the Norway lobster (Nephrops norvegicus) gut bacterial communities
Article first published online: 10 SEP 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 74, Issue 2, pages 472–484, November 2010
How to Cite
Meziti, A., Ramette, A., Mente, E. and Kormas, K. Ar. (2010), Temporal shifts of the Norway lobster (Nephrops norvegicus) gut bacterial communities. FEMS Microbiology Ecology, 74: 472–484. doi: 10.1111/j.1574-6941.2010.00964.x
- Issue published online: 12 OCT 2010
- Article first published online: 10 SEP 2010
- Accepted manuscript online: 12 AUG 2010 12:00AM EST
- Received 7 March 2010; revised 20 July 2010; accepted 30 July 2010.Final version published online 10 September 2010.
- 16S rRNA gene;
- Nephrops norvegicus
The aim of this study was to investigate the gut bacterial communities of Nephrops norvegicus individuals, using a suite of molecular tools consisting of automated ribosomal intergenic spacer analysis, 16S rRNA gene–internal transcribed spacer clone libraries and FISH. The animals were collected from Pagasitikos Gulf, Greece, during different months of the year. The diversity of the gut bacterial communities was found to mostly vary with sampling time, which could be related to temporal variations in food supply. The 16S rRNA gene diversity analysis showed dominance of specific phylotypes for each month studied. February, May, July, August and October samples were rich in sequences related to the gammaproteobacterial genera Pseudoalteromonas, Psychrobacter and Photobacterium. September and December samples were dominated by phylotypes affiliated with uncultured representatives of Mollicutes, which are generally associated with the intestinal tracts of various animals. The presence of Gammaproteobacteria and uncultured Mollicutes in August and September samples, respectively, was further confirmed by FISH. None of the morphometric parameters considered was related to the temporal pattern of dominant bacterial communities.