Editor: Gary King
Halomonas and Marinobacter ecotypes from hydrothermal vent, subseafloor and deep-sea environments
Article first published online: 9 NOV 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 75, Issue 1, pages 123–133, January 2011
How to Cite
Kaye, J. Z., Sylvan, J. B., Edwards, K. J. and Baross, J. A. (2011), Halomonas and Marinobacter ecotypes from hydrothermal vent, subseafloor and deep-sea environments. FEMS Microbiology Ecology, 75: 123–133. doi: 10.1111/j.1574-6941.2010.00984.x
- Issue published online: 6 DEC 2010
- Article first published online: 9 NOV 2010
- Accepted manuscript online: 13 OCT 2010 08:45AM EST
- Received 8 June 2010; revised 17 September 2010; accepted 26 September 2010.Final version published online 9 November 2010.
- metal cycling
Moderately halophilic and euryhaline bacteria are routinely found in cool to warm hydrothermal vent and nearby cold, deep-sea environments. To elucidate the diversity of these microorganisms – with the goal of determining which among them constitute ecotypes specifically associated with hydrothermal vent and subseafloor habitats – PCR primers were designed to detect natural populations of euryhaline Gammaproteobacteria belonging to the cosmopolitan genera Halomonas and Marinobacter. The distribution patterns of 16S rRNA gene sequence data revealed that Halomonas group 2A comprised a subseafloor population at Axial Seamount on the Juan de Fuca Ridge. Complementary biogeographic and physiological data suggested that other Halomonas clades include members that are cold adapted (Halomonas group 2B) or associated with massive sulfide deposits (Halomonas group 2C). Similarly, a monophyletic Marinobacter clade may represent Fe2+-oxidizing facultative chemoautotrophs based on the phylogenetic data presented here and previously reported phenotypic characterizations. The biogeographic distributions of Halomonas and Marinobacter isolates and clones reveal that these are cosmopolitan genera, commonly found in the deep sea and in hydrothermal vent settings. As such, they are good candidates for further laboratory investigations into the biogeochemical processes in these environments.