Editor: Julian Marchesi
Quorum quenching in cultivable bacteria from dense marine coastal microbial communities
Article first published online: 13 DEC 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 75, Issue 2, pages 205–217, February 2011
How to Cite
Romero, M., Martin-Cuadrado, A.-B., Roca-Rivada, A., Cabello, A. M. and Otero, A. (2011), Quorum quenching in cultivable bacteria from dense marine coastal microbial communities. FEMS Microbiology Ecology, 75: 205–217. doi: 10.1111/j.1574-6941.2010.01011.x
- Issue published online: 4 JAN 2011
- Article first published online: 13 DEC 2010
- Accepted manuscript online: 16 NOV 2010 09:10AM EST
- Received 24 August 2010; revised 2 November 2010; accepted 8 November 2010., Final version published online 13 December 2010.
- quorum sensing;
- marine bacteria
Acylhomoserine lactone (AHLs)-mediated quorum-sensing (QS) processes seem to be common in the marine environment and among marine pathogenic bacteria, but no data are available on the prevalence of bacteria capable of interfering with QS in the sea, a process that has been generally termed ‘quorum quenching’ (QQ). One hundred and sixty-six strains isolated from different marine dense microbial communities were screened for their ability to interfere with AHL activity. Twenty-four strains (14.4%) were able to eliminate or significantly reduce N-hexanoyl-l-homoserine lactone activity as detected by the biosensor strain Chromobacterium violaceum CV026, a much higher percentage than that reported for soil isolates, which reinforces the ecological role of QS and QQ in the marine environment. Among these, 15 strains were also able to inhibit N-decanoyl-l-homoserine lactone activity and all of them were confirmed to enzymatically inactivate the AHL signals by HPLC-MS. Active isolates belonged to nine different genera of prevalently or exclusively marine origin, including members of the Alpha- and Gammaproteobacteria (8), Actinobacteria (2), Firmicutes (4) and Bacteroidetes (1). Whether the high frequency and diversity of cultivable bacteria with QQ activity found in near-shore marine isolates reflects their prevalence among pelagic marine bacterial communities deserves further investigation in order to understand the ecological importance of AHL-mediated QS and QQ processes in the marine environment.