Editor: Christoph Tebbe
Effect of heat shock treatment on stress tolerance and biocontrol efficacy of Metschnikowia fructicola
Article first published online: 28 JAN 2011
FEMS Microbiology Ecology © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. No claim to original US government works
FEMS Microbiology Ecology
Volume 76, Issue 1, pages 145–155, April 2011
How to Cite
Liu, J., Wisniewski, M., Droby, S., Tian, S., Hershkovitz, V. and Tworkoski, T. (2011), Effect of heat shock treatment on stress tolerance and biocontrol efficacy of Metschnikowia fructicola. FEMS Microbiology Ecology, 76: 145–155. doi: 10.1111/j.1574-6941.2010.01037.x
- Issue published online: 7 MAR 2011
- Article first published online: 28 JAN 2011
- Accepted manuscript online: 11 JAN 2011 08:59AM EST
- Received 18 August 2010; revised 12 December 2010; accepted 14 December 2010., Final version published online 28 January 2011.
- heat tolerance;
- oxidative stress tolerance;
- postharvest disease
The effect of high temperature and oxidative stress on the cell viability of the yeast antagonist, Metschnikowia fructicola was determined. A mild heat shock (HS) pretreatment (30 min at 40 °C) improved the tolerance of M. fructicola to subsequent high temperature (45 °C, 20–30 min) and oxidative stress (0.4 mol L−1 hydrogen peroxide, 20–60 min). HS-treated yeast cells showed less accumulation of reactive oxygen species (ROS) than nontreated cells in response to both stresses. Additionally, HS-treated yeast exhibited significantly greater (P<0.0001) biocontrol activity against Penicillium expansum and a significantly faster (P<0.0001) growth rate in wounds of apple fruits stored at 25 °C compared with the performance of untreated yeast cells. Transcription of a trehalose-6-phosphate synthase gene (TPS1) was upregulated in response to HS and trehalose content also increased. Results indicate that the higher levels of trehalose induced by the HS may contribute to an improvement in ROS scavenging, stress tolerance, population growth in apple wounds and biocontrol activity of M. fructicola.