Editor: Kornelia Smalla
Genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (Glycine soja Sieb. & Zucc.) in different ecoregions of China
Article first published online: 14 MAR 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Ecology
Volume 76, Issue 3, pages 439–450, June 2011
How to Cite
Wu, L. J., Wang, H. Q., Wang, E. T., Chen, W. X. and Tian, C. F. (2011), Genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (Glycine soja Sieb. & Zucc.) in different ecoregions of China. FEMS Microbiology Ecology, 76: 439–450. doi: 10.1111/j.1574-6941.2011.01064.x
- Issue published online: 6 MAY 2011
- Article first published online: 14 MAR 2011
- Accepted manuscript online: 9 FEB 2011 03:53AM EST
- Received 16 June 2010; revised 10 November 2010; accepted 26 January 2011., Final version published online 14 March 2011.
- geographic distribution;
- Glycine soja;
A total of 99 bacterial isolates that originated from root nodules of Glycine soja were characterized with restriction analyses of amplified 16S ribosomal DNA and 16S–23S rDNA intergenic spacers (ITS), and sequence analyses of 16S rRNA, rpoB, atpD, recA and nodC genes. When tested for nodulation of G. soja, 72 of the isolates were effective symbionts, and these belonged to five species: Bradyrhizobium japonicum, Bradyrhizobium elkanii, Bradyrhizobium yuanmingense, Bradyrhizobium liaoningense and Sinorhizobium fredii. All of these, except some B. yuanmingense strains, also formed effective nodules on the domesticated soybean Glycine max. The remaining 27 isolates did not nodulate either host, but were identified as Rhizobium. Phylogeny nodC in the G. soja symbionts suggested that this symbiosis gene was mainly maintained by vertical gene transfer. Different nodC sublineages and rrs-ITS clusters reflected the geographic origins of isolates in this study.