Distribution and diversity of archaeal communities in selected Chinese soils

Authors

  • Peng Cao,

    1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
    2. Graduate School, Chinese Academy of Sciences, Beijing, China
    Search for more papers by this author
  • Li-Mei Zhang,

    1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
    Search for more papers by this author
  • Ju-Pei Shen,

    1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
    Search for more papers by this author
  • Yuan-Ming Zheng,

    1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
    Search for more papers by this author
  • Hong J. Di,

    1. Centre for Soil and Environmental Research, Lincoln University, Lincoln, Christchurch, New Zealand
    Search for more papers by this author
  • Ji-Zheng He

    Corresponding author
    • State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
    Search for more papers by this author

Correspondence: Ji-Zheng He, State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Tel.: +86 10 62849788; fax: +86 10 62923563; e-mail: jzhe@rcees.ac.cn

Abstract

To understand the distribution and diversity of archaea in Chinese soils, the archaeal communities in a series of topsoils and soil profiles were investigated using quantitative PCR, T-RFLP combining sequencing methods. Archaeal 16S rRNA gene copy numbers, ranging from 4.96 × 106 to 1.30 × 108 copies g−1 dry soil, were positively correlated with soil pH, organic carbon and total nitrogen in the topsoils. In the soil profiles, archaeal abundance was positively correlated with soil pH but negatively with depth profile. The relative abundance of archaea in the prokaryotes (sum of bacteria and archaea) ranged from 0.20% to 9.26% and tended to increase along the depth profile. T-RFLP and phylogenetic analyses revealed that the structure of archaeal communities in cinnamon soils, brown soils, and fluvo-aquic soils was similar and dominated by Crenarchaeota group 1.1b and 1.1a. These were different from those in red soils, which were dominated by Crenarchaeota group 1.3 and 1.1c. Canonical correspondence analysis indicated that the archaeal community was primarily influenced by soil pH.

Ancillary