Editor: Patrik Bavoil
Inactivation of the group A Streptococcus regulator srv results in chromosome wide reduction of transcript levels, and changes in extracellular levels of Sic and SpeB
Article first published online: 25 SEP 2006
FEMS Immunology & Medical Microbiology
Volume 48, Issue 2, pages 283–292, November 2006
How to Cite
Reid, S. D., Chaussee, M. S., Doern, C. D., Chaussee, M. A., Montgomery, A. G., Sturdevant, D. E. and Musser, J. M. (2006), Inactivation of the group A Streptococcus regulator srv results in chromosome wide reduction of transcript levels, and changes in extracellular levels of Sic and SpeB. FEMS Immunology & Medical Microbiology, 48: 283–292. doi: 10.1111/j.1574-695X.2006.00150.x
- Issue published online: 25 SEP 2006
- Article first published online: 25 SEP 2006
- Received 5 December 2005; revised 7 June 2006; accepted 19 July 2006.First published online 25 September 2006.
- group A Streptococcus;
- virulence gene regulation;
- streptococcal regulator of virulence;
- Streptococcus pyogenes
Group A Streptococcus is characterized by the ability to cause a diverse number of human infections including pharyngitis, necrotizing fasciitis, toxic shock syndrome, and acute rheumatic fever, yet the regulation of streptococcal genes involved in disease processes and survival in the host is not completely understood. Genome scale analysis has revealed a complex regulatory network including 13 two-component regulatory systems and more than 100 additional putative regulators, the majority of which remain uncharacterized. Among these is the streptococcal regulator of virulence, Srv, the first Group A Streptococcus member of the Crp/Fnr family of transcriptional regulators. Previous work demonstrated that the loss of srv resulted in a significant decrease in Group A Streptococcus virulence. To begin to define the gene products influenced by Srv, we combined microarray and two-dimensional gel electrophoresis analysis. Loss of srv results in a chromosome wide reduction of gene transcription and changes in the production of the extracellular virulence factors Sic (streptococcal inhibitor of complement) and SpeB (cysteine proteinase). Sic levels are reduced in the srv mutant, whereas the extracellular concentration and activity of SpeB is increased. These data link Srv to the increasingly complex GAS regulatory network.