Editor: Jennelle Kyd
Role of Toll-like receptor 4 in innate immune responses in a mouse model of acute otitis media
Version of Record online: 8 JAN 2007
FEMS Immunology & Medical Microbiology
Volume 49, Issue 1, pages 75–83, February 2007
How to Cite
Hirano, T., Kodama, S., Fujita, K., Maeda, K. and Suzuki, M. (2007), Role of Toll-like receptor 4 in innate immune responses in a mouse model of acute otitis media. FEMS Immunology & Medical Microbiology, 49: 75–83. doi: 10.1111/j.1574-695X.2006.00186.x
- Issue online: 24 JAN 2007
- Version of Record online: 8 JAN 2007
- Received 11 March 2006; revised 20 July 2006; accepted 18 October 2006.First published online January 2007.
- Toll-like receptor 4;
- acute otitis media;
- Haemophilus influenzae;
- polymorphonuclear cell;
- intracellular adhesion molecule-1
Nontypeable Haemophilus influenzae (NTHi) is considered a major pathogen underlying middle ear infection. This study characterized the role of Toll-like receptor 4 in the innate immune responses to acute otitis media induced by NTHi in mice. We used C3H/HeJ mice, which have nonfunctional Toll-like receptor 4, and normal wild-type C3H/HeN mice. NTHi were injected into the tympanic bulla, and middle ear effusions and tissues were collected. In C3H/HeN mice, the severity of acute otitis media decreased promptly with a significant reduction in bacterial recovery from middle ear effusions 48 h after injection. In contrast, all C3H/HeJ mice had otitis media at all time points examined, and increasing bacterial counts from middle ear effusions were detected in C3H/HeJ mice 72 h after injection. Expression of intracellular adhesion molecule-1 by the middle ear mucosa paralleled the number of polymorphonuclear cells in the middle ear in both strains. The findings of transmission electron microscopy revealed that phagocytosis and phagosome maturation of polymorphonuclear cells was impaired in C3H/HeJ mice. Our findings indicate that Toll-like receptor 4 plays a part in the early accumulation and functional promotion of polymorphonuclear cells in the middle ear for eradicating NTHi infection.