Editor: Alex van Belkum
Interaction of adrenomedullin and calcitonin gene-related peptide with the periodontal pathogen Porphyromonas gingivalis
Article first published online: 8 JAN 2007
FEMS Immunology & Medical Microbiology
Volume 49, Issue 1, pages 91–97, February 2007
How to Cite
Allaker, R. P., Sheehan, B. E., McAnerney, D. C. and McKay, I. J. (2007), Interaction of adrenomedullin and calcitonin gene-related peptide with the periodontal pathogen Porphyromonas gingivalis. FEMS Immunology & Medical Microbiology, 49: 91–97. doi: 10.1111/j.1574-695X.2006.00202.x
- Issue published online: 24 JAN 2007
- Article first published online: 8 JAN 2007
- Received 21 September 2006; revised 3 November 2006; accepted 27 November 2006.First published online January 2007.
- Porphyromonas gingivalis;
- innate immunity;
The nature of the interaction between Porphyromonas gingivalis and the multifunctional peptides adrenomedullin and calcitonin gene-related peptide (CGRP) was investigated. Growth of P. gingivalis was not inhibited in the presence of either of these peptides [minimal inhibitory concentration (MIC)>250 μg mL−1]. The ability of the arginine- and lysine-specific proteases from P. gingivalis to breakdown these peptides was investigated. Adrenomedullin and CGRP were incubated with culture supernatants from wild-type and protease gene knockout strains. No significant effect on antimicrobial activity against the indicator organism Escherichia coli BUE55 was found (MIC=6.25 μg mL−1 in all cases). The role of anionic components on the surface of P. gingivalis, which may alter binding of these cationic peptides, was also investigated in relation to adrenomedullin. Growth of gene knockout strains lacking surface polysaccharide and capsule components was not inhibited (MIC>250 μg mL−1). It is suggested that a lack of sensitivity to adrenomedullin and CGRP may enable P. gingivalis to persist in the oral cavity and cause disease.