Editor: Johannes Kusters
Antiadhesive property of microalgal polysaccharide extract on the binding of Helicobacter pylori to gastric mucin
Article first published online: 22 MAY 2007
FEMS Immunology & Medical Microbiology
Volume 50, Issue 2, pages 231–238, July 2007
How to Cite
Loke, M. F., Lui, S. Y., Ng, B. L., Gong, M. and Ho, B. (2007), Antiadhesive property of microalgal polysaccharide extract on the binding of Helicobacter pylori to gastric mucin. FEMS Immunology & Medical Microbiology, 50: 231–238. doi: 10.1111/j.1574-695X.2007.00248.x
- Issue published online: 22 MAY 2007
- Article first published online: 22 MAY 2007
- Received 15 September 2006; revised 12 February 2007; accepted accepted 14 March 2007.First published online 22 May 2007.
- Helicobacter pylori;
- microalgal polysaccharide;
- gastric mucin;
- alkyl hydroperoxide reductase
The emergence of antibiotic-resistant Helicobacter pylori is of concern in the treatment of H. pylori-associated gastroduodenal diseases. As the organism was reported to bind gastric mucin, we used porcine gastric mucin as substrate to assess the antiadhesive property of polysaccharides derived from Spirulina (PS), a commercially available microalga, against the binding of H. pylori to gastric mucin. Results show that polysaccharides prevented H. pylori from binding to gastric mucin optimally at pH 2.0, without affecting the viability of either bacteria or gastric epithelial cells, thus favouring its antiadhesive action in a gastric environment. Using ligand overlay analysis, polysaccharide was demonstrated to bind H. pylori alkyl hydroperoxide reductase (AhpC) and urease, which have shown here to possess mucin-binding activity. An in vivo study demonstrated that bacteria load was reduced by >90% in BALB/c mice treated with either Spirulina or polysaccharides. It is thus suggested that polysaccharides may function as a potential antiadhesive agent against H. pylori colonization of gastric mucin.