Editor: Artur Ulmer
Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis
Article first published online: 20 AUG 2007
FEMS Immunology & Medical Microbiology
Volume 51, Issue 2, pages 302–309, November 2007
How to Cite
Panaro, M. A., Cianciulli, A., Mitolo, V., Mitolo, C. I., Acquafredda, A., Brandonisio, O. and Cavallo, P. (2007), Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis. FEMS Immunology & Medical Microbiology, 51: 302–309. doi: 10.1111/j.1574-695X.2007.00304.x
- Issue published online: 20 AUG 2007
- Article first published online: 20 AUG 2007
- Received 11 April 2007; revised 31 May 2007; accepted 22 June 2007.First published online October 2007.
- Giardia intestinalis;
- HCT-8 cell;
Giardia intestinalis is a flagellated protozoan which causes enteric disease worldwide. Giardia trophozoites infect epithelial cells of the proximal small intestine and can cause acute or chronic diarrhea. The mechanism of epithelial injury in giardiasis remains unknown. A number of enteric pathogens, including protozoan parasites, are able to induce enterocyte apoptosis. The aim of this work was to assess whether G. intestinalis strain WB clone C6 is able to induce apoptosis in the human intestinal epithelial cell line HCT-8, and to investigate the role of caspases in this process. Results demonstrated that the parasite induces cell apoptosis, as confirmed by DNA fragmentation analysis, detection of active caspase-3 and degradation of the caspase-3 substrate PARP [poly(ADP-ribose) polymerase]. Furthermore, G. intestinalis infection induces activation of both the intrinsic and the extrinsic apoptotic pathways, down-regulation of the antiapoptotic protein Bcl-2 and up-regulation of the proapoptotic Bax, suggesting a possible role for caspase-dependent apoptosis in the pathogenesis of giardiasis.