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Keywords:

  • M-cells;
  • translocation;
  • bacteria;
  • pathogens

Abstract

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

M-cells are specialized cells found in the follicle-associated epithelium of intestinal Peyer's patches of gut-associated lymphoid tissue and in isolated lymphoid follicles, appendix and in mucosal-associated lymphoid tissue sites outside the gastrointestinal tract. In the gastrointestinal tract, M-cells play an important role in transport of antigen from the lumen of the small intestine to mucosal lymphoid tissues, where processing and initiation of immune responses occur. Thus, M-cells act as gateways to the mucosal immune system and this function has been exploited by many invading pathogens. Understanding the mechanism by which M-cells sample antigen will inform the design of oral vaccines with improved efficacy in priming mucosal and systemic immune responses. In this review, the origin and morphology of M-cells, and their role in mucosal immunity and pathogenesis of infections are discussed.


Introduction

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

The mucosal-associated lymphoid tissue (MALT), consisting of immunoreactive cells and organized lymphoid tissues, is found in close contact with all mucosa throughout the body. In the intestine, it is termed gut-associated lymphoid tissue (GALT), which consists of both isolated and aggregated lymphoid follicles (Neutra et al., 2001). These are the sites where antigen recognition and mucosal immune responses are initiated. Aggregated lymphoid follicles are found in Peyer's patches (PP) of the small intestine, appendix vermiformis, and caecum, colon and rectum patches. GALT is one of the largest lymphoid organs in the body, containing up to 70% of the body's immunocytes. Typical GALT structures can be seen in PP, aggregated lymphoid follicles in the small intestinal mucosa. PP are named after the 17th-century Swiss anatomist Hans Conrad Peyer and are located along the antimesenteric side of the small intestine. Morphologically, PP are separated into three main domains: the follicular area, the parafollicular area and the follicle-associated epithelium (Neutra et al., 2001). The follicular and parafollicular areas consist of the PP lymphoid follicle, which has a germinal centre containing proliferating B-lymphocytes, follicular dendritic cells (FDC) and macrophages. The follicle is surrounded by the corona, containing small lymphocytes expressing IgM and IgD; a dome lies above the follicle and contains B- and T-lymphocytes, dendritic cells (DCs) and macrophages. The follicle-associated epithelium (FAE) is a one-cell-thick layer composed of enterocytes and specialized epithelial cells termed M-cells (Owen & Jones, 1974). The FAE overlies the PP and forms the interface between the intestinal lymphoid system and the intestinal luminal environment. The function of PP was unknown until 1922, when Kenzaburo Kumagai identified uptake of Mycobacterium tuberculosis at the dome epithelium. However, he also observed uptake of heat-killed bacteria, sheep red blood cells and India ink by PP and hence concluded that this uptake was a nonspecific process. With the development of techniques for ultrastructural analysis in 1972, M-cells capable of taking up antigen were identified and the role of PP in the immune system became clear (Owen & Jones, 1974).

M-cells

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

M-cells are specialized epithelial cells found in the FAE of PP, and in isolated lymphoid follicles, appendix, and in MALT sites outside the gastrointestinal tract. M-cells differ morphologically and enzymatically from adjacent enterocytes. In 1965, M-cells were first identified in rabbit appendix by J.F. Schmedtje (Owen & Jones, 1974). They were initially called lymphoepithelial cells but were later renamed M-cells, when Owen & Jones (1974) used electron microscopy to study PP of human small intestine and found the presence of ‘microfolds’ on the apical surface of these epithelial cells. M-cells act as gatekeepers to the mucosal immune system, continuously sampling the lumen of the small intestine and transporting antigen to the underlying mucosal lymphoid tissue for processing and initiation of immune responses (Kraehenbuhl & Neutra, 1992; Neutra et al., 1996a, b). This M-cell sampling process has been exploited as a means of translocating the epithelium by pathogens including Salmonella typhimurium (Jensen et al., 1998).

Origin and development of M-cells

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

The origin of M-cells within the FAE remains unclear and is the subject of much debate. It is known that intestinal epithelial cells in the FAE originate from stem cells in crypts located between a villus and a PP dome. Each crypt harbours a ring of stem cells that generate distinct cell types and there are two distinct axes of migration and differentiation (Heath, 1996). Cells located on the villous side of the crypt differentiate into absorptive enterocytes, goblet cells and endocrine cells as they migrate upwards in columns along the villous (Sierro et al., 2000). Cells enter programmed cell death as they reach the tip of the villous and are shed into the intestinal lumen (Sierro et al., 2000). Cells on the FAE side of the crypt move into the dome and differentiate into absorptive enterocytes and M-cells (Garvrieli et al., 1992).

While it is accepted that enterocytes and M-cells have a common precursor, it is not known whether crypt cells commit early to FAE and M-cell phenotypes, or whether factors act later to induce further differentiation of enterocytes into M-cells (Nicoletti, 2000). Certainly, an established in vitro coculture system demonstrates that human intestinal enterocytes are converted to M-like cells by murine Peyer's patch lymphocytes (PPL) (Kerneis et al., 1997). In this in vitro model, PPL migrate into spaces between epithelial cells, forming intraepithelial pockets in enterocytes with disordered apical microvilli, characteristic features of an M-cell (Kerneis et al., 1997). However, whether this reflects the mechanisms governing in vivo differentiation is unclear as the in vitro system utilizes adenocarcinoma cells (Caco-2) that do not behave like normal enterocytes. Furthermore, a recent study found that prior treatment of Caco-2 epithelial monolayers with a human lymphocyte cell line is not absolutely required for differentiation to the M-like cell phenotype (Blanco & DiRita, 2006a). The presence of M-like cells in untreated Caco-2 monolayers was confirmed by the observation of cells lacking an organized brushborder and the presence of the M-cell marker, sialyl-Lewis A antigen (Blanco & DiRita, 2006a).

Combining histochemical and ultrastructural techniques, Gebert et al. (1999) analysed PP dome epithelium development and found M-cell precursor cells in specialized dome-associated crypts. These crypts differ from ordinary crypts in size, shape, cellular composition and location. Thus, this study suggests that M-cell differentiation is restricted to specialized crypts and is not induced by lymphocytes. Furthermore, a study by Gebert et al. (1999) detected M-cell precursors in the early proliferative zone of the crypts using M-cell-specific monoclonal antibodies. This observation again supports the theory that M-cells arise from a distinct cell lineage.

M-cell morphology

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

M-cells are distinguished from intestinal enterocytes by distinctive morphological features. At the apical surface, M-cells display a poorly organized brush border with short irregular microvilli, in contrast to the highly organized brush border of enterocytes, with uniform densely packed microvilli (Figs 1 and 2) (Kerneis et al., 1997). Immunostaining of the microvillar proteins, actin and villin, has been used to identify M-cells that are detected by the absence of staining due to their short, irregular microvilli (Kanaya et al., 2007). The usually thick glycocalyx associated with absorptive cells is absent in M-cells and is replaced by a thin glycocalyx, which is thought to aid greater access to antigens in the gut lumen. M-cells lack certain enterocyte apical surface glycoproteins. These include alkaline phosphatase and sucrase-isomaltase activity, which are typical to the brushborder of enterocytes, and both have been used as negative markers for M-cells (Gebert et al., 1996). A lack of alkaline phosphatase has been used to signify M-cells in mice, rabbits, rats, dogs and humans (Gebert et al., 1996). At their basolateral surface, M-cells possess a unique intraepithelial invagination or ‘pocket’ (Neutra et al., 1996a). The M-cell pocket contains B- and T-lymphocytes, macrophages and DCs. It provides a docking site for lymphocytes and other antigen-presenting cells, reducing the distance from the apical to the basolateral surface that transcytotic vesicles travel (Trier, 1991). The basolateral surface of epithelial cells contains two major subdomains: the lateral subdomain is involved in cell–cell adhesion and contains Na+–K+–ATPase, and the basal subdomain interacts with the extracellular matrix and the lamina propria (Trier, 1991).

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Figure 1.  Electronmicrograph of an M-cell from a Caco-2 coculture experiment displaying short irregular microvilli (Corr et al., 2006).

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Figure 2.  Overview of M-cell location within the PP FAE.

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While little is known about the glycoproteins or adhesion molecules expressed on the apical surface of M-cells, M-cells have been shown to display distinct glycosylation patterns as compared with their enterocyte neighbours (Gebert & Hach, 1993). Lectins have been used to characterize this glycosylation pattern. Ulex europaeus 1 (UEA-1) and Psophocarpus tetragonolobus (WBA) are two lectins that have been used to stain murine M-cells (Giannasca et al., 1994). UEA-1 is specific for carbohydrate structures that contain α-1-fucose residues, and specifically stains the apical surfaces of M-cells (Giannasca et al., 1999). These lectin-binding sites were also observed on M-cell plasma membranes, including the basolateral membrane, pocket domain and on intracellular vesicles (Neutra et al., 1999). Furthermore, glycosylation patterns vary between M-cells within a single FAE, at different intestinal locations and between species (Gebert & Hach, 1993). Rabbit caecal M-cells selectively bind UEA-1 but UEA-1 is not bound by rabbit PP and M-cells in mouse caecal patches (Jepson et al., 1996). Also, human colonic M-cells express intercellular adhesion molecule 1 (ICAM-1) but it is not expressed by PP M-cells (Jepson et al., 1996).

Research is ongoing to determine the cellular markers of human M-cells. Significantly, recent work has utilized microarray analysis of Caco-2 cells differentiated to M-like cells and determined 180 differentially regulated genes. Of these potential targets, the molecule Galectin 9 was shown to be expressed on the apical surface of M-like cells and FAE and may provide a molecular marker of human M-cell differentiation (Pielage et al., 2007). Monoclonal antibodies that are specific against single carbohydrate epitopes have also been used to identify M-cells in humans (Neutra et al., 1999). Using this approach, M-cells have been shown to express sialyl Lewis antigen on their apical and subcellular membranes (Giannasca et al., 1999).

M-cell function

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

The reduced brush border and lack of enzymatic activity of the M-cell apical surface suggests that they are unlikely to play a role in digestion or absorption. The exposed nature of the M-cell apical surface instead indicates that the primary function of M-cells is trans-epithelial transport. M-cells transport substances from the lumen of the intestine, across the epithelial barrier and to underlying immune cells where processing and initiation of immune responses occur. M-cells have been shown to transport particulates including latex beads, carbon particles and liposomes and macromolecules including ferritin, horseradish peroxidase, cholera toxin-binding subunit, lectins and antivirus antibodies (Gebert et al., 1996). M-cells have also been shown to transport microorganisms including Vibrio cholerae and S. typhimurium in vitro (Kerneis et al., 1997; Jensen et al., 1998). This transport process occurs via trans-cellular endocytosis and has been shown to be temperature-dependent, transport being inhibited at 25 °C or lower (DesRieux et al., 2005).

The trans-epithelial transport process occurs in three stages. Firstly, endocytosis of the substance occurs at the apical membrane, followed by transport of the substance via an endocytic vesicle to the endosomal compartment, and finally exocytosis at the basolateral membrane (DesRieux et al., 2005). M-cell-mediated-translocation is very efficient and is a rapid process. As the M-cell cytoplasm above the pocket is a thin apical rim, a complete endocytosis–exocytosis sequence can take a minimum of ten minutes (Kraehenbuhl & Neutra, 1992). The mechanisms by which M-cells take up microorganisms and molecules vary according to the nature of the material such as size, local surface pH, surface charge, hydrophobicity, concentration and the presence or absence of an M-cell-specific receptor (DesRieux et al., 2005). For example, large particles and bacteria can induce phagocytosis associated with apical membrane ruffling and rearrangement of the actin cytoskeleton (Liang et al., 2001). Viruses and other adherent particles are taken up by endocytosis via clathrin-coated vesicles (Owen, 1977). Nonadherent material is taken up by fluid-phase endocytosis and this has been shown in the case of soluble tracers such as ferritin (Neutra et al., 1987). A study by Neutra et al. (1987) found that a membrane-bound tracer, wheat germ agglutinin (WGA) lectin, is transported by M-cells c. 50 times more efficiently than the soluble tracer, bovine serum albumin (BSA).

During transcytosis, antigens do not undergo major ultrastructural alteration and are released intact into the pocket. While M-cells have a 16-fold decrease in lysosome volume and reduced lysozyme (acid phosphatase) activity compared with normal enterocytes, there is some evidence that M-cells may possess some enzymatic activity. Acid phosphatase-enriched prelysosomes, lysosome vesicles and major-histocompatability complex (MHC) class II determinants in the basolateral domain were detected in rat M-cells, suggesting a role in antigen processing and presentation (Allan et al., 1993). The aspartic proteinase Cathepsin E, normally found in the lysosomal compartment of antigen-presenting cells, has been localized in human and rat M-cells, again suggesting a role in processing and presentation (Finzi et al., 1993). Despite these observations, the extent of degradation and participation of M-cells in antigen processing and presentation remains unclear.

M-cells have other roles apart from antigen transport. It has been suggested that M-cells may aid immune response induction to the antigen they are transporting by releasing a costimulatory signal for T- and B-cell proliferation (Pappo & Mahlman, 1993). M-cells isolated from rabbit FAE were shown to secrete IL-1 following lipopolysaccharide stimulation in vitro and these culture supernatants were shown to induce T-cell proliferation (Pappo & Mahlman, 1993). This suggests that transcytosis of bacteria results in bacterial-lipopolysaccharide-induced IL-1 release, aiding proliferation of lymphocytes for induction of a mucosal immune response.

Entry sites for pathogens

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

M-cells are the main sites for continuous sampling and transport of antigens from the intestinal lumen to mucosal lymphoid tissues. Despite the production of antimicrobial peptides at the M-cell gateway (Goitsuka et al., 2007), this region of the FAE can be considered to be a potential ‘Achilles heel’ in the mucosal barrier and M-cells are exploited by many pathogens as a route of entry to underlying host tissues (Sansonetti & Phalipon, 1999). These include Poliovirus, S. typhimurium, Yersinia enterocolitica and V. cholera (Kerneis et al., 1997; Jensen et al., 1998; Ouzilou et al., 2002; Hamzaoui et al., 2004). As M-cells are capable of transporting inert particles, it has been suggested that pathogens may interact with M-cells via nonspecific passive mechanisms. However, several studies indicate that specific mechanisms of interaction mediate transport of microorganisms by M-cells (Tyrer et al., 2007). Understanding the mechanisms by which some microorganisms selectively use M-cells to cross the intestinal epithelium may aid development of disease control strategies. It may also allow exploitation of these invasion strategies for mucosal drug and vaccine delivery (Brayden et al., 2005).

Salmonella

Salmonella spp. are probably the most-studied pathogens that translocate M-cells. Murine ligated loop models have shown that M-cells are the major route of entry for Salmonella (Jones et al., 1994). Salmonella typhimurium exhibits selective targeting of M-cells overlying PP and can induce uptake, which is associated with extensive ruffling and FAE damage (Sansonetti & Phalipon, 1999). Adherence and invasion of M-cells induces apical membrane ruffling and actin polymerization, leading to bacterial engulfment, M-cell cytotoxicity and sloughing of the FAE (Jones et al., 1994). This FAE damage could potentially allow unrestricted bacterial invasion and may explain the occurrence of intestinal ulcers and perforations in typhoid patients (Jones et al., 1994). Rearrangements of FAE were exclusively observed at sites of M-cells in mice 30 min postinfection with S. typhimurium, with the integrity of enterocytes remaining intact. With increased length of exposure, increased M-cell membrane alterations were observed, with most M-cells displaying membrane protrusions and disruptions after 90 min. These disruptions result in pores in the epithelium that allow bacterial spread to organs before an immune response is established (Jones et al., 1994). The invasion machinery, encoded by genes located on the Salmonella pathogenicity island 1 (SPI1), is crucial for invasion of epithelial cells and also plays a role in M-cell invasion (Clark et al., 1998b). However, while mutants in SPI1 still invade M-cells they, are not cytotoxic and do not result in the destruction of the FAE (Jones et al., 1994). Salmonella typhimurium expresses a number of fimbrial operons, including fim, lpf and pef. These mediate binding to different murine epithelial cells and the lpf operon appears to be responsible for specific adherence to murine M-cells (Baumler et al., 1996). An S. typhimurium mutant in lpfC exhibited reduced colonization of PP and reduced destruction of M-cells, suggesting that S. typhimurium target M-cells via these fimbriae (Baumler et al., 1996). Following transport across the FAE, Salmonella are phagocytosed by macrophages and DCs where they can survive within the phagocytic vacuole due to genes encoded by a second pathogenicity island (SPI2) (Baumler et al., 1996).

Yersinia

Microscopic studies in mice have shown that Yersinia enterocolitica and Yersinia pseudotuberculosis adhere to both enterocytes and M-cells, but demonstrate a preference for M-cells (Autenrieth & Firsching, 1996; Marra & Isberg, 1997; Sansonetti & Phalipon, 1999). Infection with Y. enterocolitica is accompanied by major damage to PP (Autenrieth & Firsching, 1996). It has been demonstrated that targeting of Yersinia to M-cells is mediated by invasin, an outer-membrane protein that binds to integrins of the β1 family expressed apically on M-cells (Autenrieth & Firsching, 1996; Marra & Isberg, 1997; Clark et al., 1998a). Despite a recent study that showed that an invasin-deficient strain can still adhere to M-cells in vitro, Yersinia mutants lacking invasin protein display reduced colonization and translocation of PPs in vivo (Marra & Isberg, 1997; Hamzaoui et al., 2004). Following M-cell uptake, bacteria must survive phagocytosis by macrophages. Yersinia have developed a mechanism of antiphagocytosis that allows them to remain extracellular (Forsberg et al., 1994). This strategy is shared by the enteropathogenic Y. enterocolitica and Y. pseudotuberculosis, and also the plague organism Yersinia pestis. Following adherence to the eukaryotic cell surface, the bacterium injects a set of Yop proteins (Yop-E, -H, -T, -O, -P and -M) into the cytoplasm via a Type III secretion system (Grosdent et al., 2002). These Yop proteins induce breakdown of the F-actin network and Yop-O, also called YpkA, interrupts signalling pathways of phagocytosis (Forsberg et al., 1994).

Shigella flexneri

Invasion of the intestinal epithelium by Shigella spp. causes the acute infection termed recto-colitis, better known as shigellosis. Inflammatory lesions occur on the upper rectum and distal colon. Upon further histopathological study, these ulcers were found to be located at sites of lymphoid follicles, suggesting that the FAE is a major route of entry (Sansonetti et al., 1996). In rabbit ligated loop assays, S. flexneri enter M-cells after a short infection period (2–8 h), causing an increase in M-cell size due to accumulation of mononuclear cells in the pocket and thus acts as an increased area for transport (Perdomo et al., 1994). This process is accompanied by extensive cellular damage and membrane ruffling; however, S. flexneri are not cytotoxic to M-cells (Perdomo et al., 1994; Jensen et al., 1998, Sansonetti & Phalipon, 1999). Following passage across the FAE, bacteria reinvade epithelial cells basolaterally and invasion is followed by an inflammatory process that disrupts the epithelium and increases permeability, facilitating further passage across the FAE (Perdomo et al., 1994). Bacteria are then engulfed by macrophages and DCs. Two to four hours following infection, bacteria induce apoptosis by expressing four ipa genes encoding secretory proteins and an Mxi/Spa secretory apparatus. Bacteria release an IpaB invasin that binds to IL-1β-converting enzyme, causing cleavage of target proteins and cell death. Upon apoptosis, bacteria are released and spread from cell to cell, inducing the production of proinflammatory cytokines, IL-8 and TNF-α by enterocytes (Sansonetti & Phalipon, 1999; Phalipon & Sansonetti, 2007).

Vibrio cholerae

Binding of V. cholerae to M-cells occurs via a tight attachment domain that induces actin filaments to form structures that engulf the bacteria. Heat-killed bacteria do not attach to M-cells, indicating that the interaction requires specific bacterial adhesions (Blanco & DiRita, 2006a). A recent study found that V. cholerae is transcytosed by M-cells via interaction of cholera toxin and ganglioside receptor, GM1 (Blanco & DiRita, 2006a, b). In this study, heat-killed bacteria were not transcytosed as heat-killing may destroy or remove cholera toxin required for binding to GM1. Following transport across M-cells, V. cholerae infect lymphoblasts and macrophages, but this is accompanied by subsequent killing of the bacteria in the follicle dome (Davis & Owen, 1997). Uptake induces a host sIgA response to cholera toxin and the cholera lipopolysaccharide (Pierce et al., 1987). However, pathogen-bound sIgA may then further enhance M-cell-mediated uptake of V. cholerae through targeting of M-cell sIgA receptors (Blanco & DiRita, 2006b).

Escherichia coli

Most strains of Escherichia coli do not adhere to M-cells, with the exception of some pathogenic strains. These include E. coli 0:124, enteroaggregative and diffuse adhering E. coli, enterotoxigenic E. coli and enteropathogenic E. coli rabbit diarrhoeagenic E. coli (RDEC)-1 (Cantey & Inman, 1981). The RDEC-1 strain of E. coli is an enteroadherent bacterium that adheres to the lymphoid follicle epithelium of ileal PP in postweanling rabbits (Inman & Cantey, 1983). In an ultrastructural study, E. coli RDEC-1 were seen to adhere specifically to M-cells of the lymphoid follicle epithelium; however, this did not result in their internalization (Cantey & Inman, 1981). Adherence is accompanied by blebbing and notching of the M-cell microvilli leading to the eventual formation of vesicles. Adherence to absorptive epithelial cells causes pedestal formation by the plasmalemma that cup the bacteria (Cantey & Inman, 1981; Marchetti et al., 2004). A recent study found that nonmotile E. coli RDEC-1 do not efficiently adhere to appendix M-cells of the rabbit (Marchetti et al., 2004). The enterohaemorrhagic E. coli strain O157:H7 selectively adheres to human FAE PP but it is not known whether it specifically adheres to M-cells. However, the protein intimin (γ), essential for its colonization, binds β1-integrins, and these are expressed on the M-cell apical surface (McKee et al., 1995; Hamzaoui et al., 2004). A recent study observed similar rates of translocation of enteropathogenic E. coli O127:H7 across an in vitro M-cell model compared with normal enterocytes (Martinez-Argudo et al., 2007). Furthermore, in this study, it was observed that translocation rates were significantly increased in the absence of a functioning TypeIII secretion system (Martinez-Argudo et al., 2007).

Listeria

Considerable research work has centred on the precise route by which the food-borne pathogen Listeria monocytogenes gains access to Intraepithelial lymphoid cells and mucosal lymphoid tissues. It has been well documented that L. monocytogenes can invade nonphagocytic cells (using bacterial internalin proteins) and that this process is critical for bacterial translocation of the intestinal epithelium (Vázquez-Boland et al., 2001; Hamon et al., 2006). Pentecost et al. (2006) have recently demonstrated that the pathogen binds to E-cadherin exposed at the villous tips during extrusion of epithelial cells in this region. Previous work has demonstrated that this interaction is host specific and that human, but not murine and rat cells are efficiently targeted by L. monocytogenes (Lecuit et al., 1999).

While it is clear that the pathogen invades host cells through a specific interaction between internalins and target receptors, some speculation surrounds whether the pathogen also has the potential to invade via M-cells for rapid translocation. This interaction would effectively prime the host immune response but could also be utilized by the pathogen as a means for accessing the underlying mucosa. Indeed, some evidence for M-cell sampling of L. monocytogenes has emerged from studies in mice and rats (Pron et al., 1998; Daniels et al., 2000). In vivo analysis of orogastric L. monocytogenes infections using animal models has demonstrated preferential replication within the PP with extremely rapid translocation of the bacterium (within 15 min) to internal organs (Pron et al., 1998). Similarly, a more recent study using the murine model of infection (Daniels et al., 2000) found very rapid translocation of Listeria from the lumen of the gastrointestinal tract to internal organs. Other studies also demonstrated rapid localization of L. monocytogenes within the PP of mice (Marco et al., 1997; Corr et al., 2006). It is evident that in the absence of a specific interaction between L. monocytogenes and murine host cells, the pathogen may be sampled within PP, providing for rapid translocation.

The in vitro model of M-cell development was recently utilized for analysis of L. monocytogenes translocation. This work demonstrates that the pathogen migrates through differentiated M-cells at rates similar to translocation of V. cholerae and more efficiently than control Lactobacillus salivarius cells. Furthermore, this interaction is independent of internalin expression or expression of the major virulence factor Listeriolysin (Corr et al., 2006). Using a similar model, Daniels et al. (2000) indicated that while L. monocytogenes attaches to and invades both enterocytes and M-cells, no preferential targeting of M-cells occurs. However, they did not examine translocation of the monolayer.

While elegant work has unravelled the specific mechanisms by which L. monocytogenes directly invades host cells (Hamon et al., 2006; Pentecost et al., 2006), there is much evidence to support a role for M-cells in sampling the pathogen from the intestinal lumen leading to rapid translocation to internal organs (Pron et al., 1998; Corr et al., 2006).

Viruses

Several viruses are transported by M-cells and reovirus type-1, poliovirus and HIV type 1 (HIV-1) bind specifically (Sicinski et al., 1990; Amerongen et al., 1991, 1994). Reovirus, an orally transmitted murine pathogen, affects the nervous system, causing encephalitis. Reovirus type-1 selectively adheres to ileal and colorectal M-cells, and this is mediated by its outer capsid proteins (Amerongen et al., 1994). Gastrointestinal transit causes proteolytic cleavage of the outer capsid proteins, which become activated for adherence (Helander et al., 2003). The receptors for reovirus type-1 are α-2-3-linked sialic acid glycoconjugates that bind the viral haemaglutinin sigma 1 (Davis & Owen, 1997). Infection causes depletion of M-cells from the FAE, which may affect host antiviral responses; however, the virus is cleared within 10 days in mice by production of antiviral sIgA (Silvey et al., 2001).

Poliovirus is the causative agent of poliomyelitis and infects humans by the oral route. It is thought that the primary sites of replication in the gut are PP (Sicinski et al., 1990). In human tissues infected with poliovirus, virions were found to be specifically adhering to the surface of M-cells and in vesicles within M-cells (Sicinski et al., 1990). Poliovirus type-1 and the corresponding attenuated vaccine strain, Sabin, both translocate across M-cells in vitro (Ouzilou et al., 2002).

Transmission of HIV-1 infection via anorectal, cervicovaginal, foreskin and urethral epithelia accounts for 80% of AIDS cases (Amerongen et al., 1991). HIV-1 must cross the mucosal barrier of the intestinal or genital tracts to infect CD4+ T-cells and translocation across epithelial cells or M-cells of FAE of intestinal and tonsil lymphoid follicles may play a role in infection (Amerongen et al., 1991). Infection is thought to occur when epithelial barriers are damaged, although studies in monkeys with simian immunodeficiency virus (SIV) have shown that infection can occur via intact rectal mucosa (Amerongen et al., 1991). HIV-1 has been shown to adhere to M-cells but not enterocytes on villi or in the FAE in rabbits and mice (Amerongen et al., 1991). Recently, it was shown that HIV-1 is transported across M-cells via lactosyl cerebroside and the chemokine receptor, CXCR4 (Fotopoulos et al., 2002). They demonstrated that a lymphotropic (X4, syncytium-inducing HIV-1) HIV-1 strain crosses M-cells via lactosyl cerebroside and CXCR4, receptors that are expressed apically on M-cells. A monotrophic (R5, nonsyncytium inducing) HIV-1 strain was unable to cross M-cells because they do not express its CCR5 receptor; however, transfection of M-cells with CCR5 cDNA restored translocation.

Prions

Prion disorders affect the mammalian brain by causing plaques and lesions. Prion diseases include scrapie in sheep, bovine spongiform encephalopathies (BSE or mad cow disease) and the human disease, Creutzfeldt–Jakob disease. These are all caused by an infectious misfolded prion (PrPSc) that converts normal cellular prions to an abnormal form (Heppner et al., 2001). Transmission of BSE to humans via dietary exposure has become a concern since the appearance of a new variant of Creutzfeld–Jakob disease (Heppner et al., 2001). Using an in vitro M-cell model, it was shown that Rocky mountain laboratory scrapie prions are transported by M-cells while no transcytosis was observed in enterocytes (Ghosh, 2002). Follicular DCs have been shown to be important for development of prion pathogenesis and subsequent infection of the neural system and this supports the role of M-cells in prion transport (Ghosh, 2002).

An in vitro M-cell model

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

The study of M-cells has been problematic due to the difficulties associated with isolating M-cells. M-cells have been difficult to identify and isolate due to their low numbers in the FAE, lack of an enriched preparation and phenotype variation between species. M-cells have been difficult to characterize biochemically and so little is known about their cell biology. In the past, studies have relied on in vivo or in situ methods such as isolated tissue loops or explant cultures. However, the development of an in vitro M-cell/FAE model has provided a reproducible approach in which phenotypic and physiological features of the FAE and M-cells overlying PP are maintained (Kerneis et al., 1997; DesRieux et al., 2007). This model facilitates the study of antigen transport across M-cells, and their interaction with lymphocytes, bacteria and vaccines (Kerneis et al., 1997). This system uses the human adenocarcinomal enterocyte-like cell line, Caco-2, to mimic M-cell activity through differentiation of epithelial enterocytes to M-cells via coculture with PPL or a Raji human lymphocyte cell line. Briefly, the M-cell model involves a two-chamber transwell system in which epithelial enterocytes are grown on a polycarbonate porous membrane until differentiation to a villous-expressing cell type. Upon differentiation, freshly isolated PPL are introduced into the basolateral chamber, where they migrate into the monolayer (Fig. 3). This coculture induces the M-cell phenotype (Kerneis et al., 1997). The efficiency of the system has been determined by its ability to mimic key M-cell properties. Translocation of fluorescein isothiocyanate (FITC)-labelled microspheres and live noninvasive V. cholerae from the apical to basolateral chamber of cocultures, are significantly increased in Caco-2 cells grown in the presence of PPL, indicative of the sampling process (Kerneis et al., 1997). Particle transport is temperature-dependent in cocultures indicating that a transcytotic route is involved (Tyrer et al., 2002). Apical expression of alkaline phosphatase is downregulated in cocultures and a redistribution of the actin-associated protein villin from the apical surface to the cytoplasm indicates a loss of an organized brushborder. α5β1 Integrin, normally expressed on the lateral and basolateral surfaces of Caco-2 cells, is present on apical membranes of cocultures (Schulte et al., 2000). The M-cell in vitro model has shown great potential in the study of the interaction of pathogens with the intestinal epithelium and also the determination of molecular mechanisms required for translocation across M-cells (Schulte et al., 2000). It also allows a method for determination of the factors and mechanisms that influence M-cell development and the development of oral vaccine delivery.

image

Figure 3.  Outline of the in vitro M-cell model (Corr et al., 2006).

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The future of M-cell research: vaccine delivery

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

Oral vaccines will have to overcome many hurdles in order to be effective. The hurdles include (1) poor accessibility of mucosal DCs due to dilution or degradation of the antigen, peristalsis and the mucus barrier, and (2) oral tolerance, which downregulates cell-mediated and humoral immune responses (Nicoletti, 2000). Given the unique features of M-cells and their specialized ability to transcytose numerous microorganisms and particulates, targeted delivery of antigens to M-cells may provide a mechanism for the efficient presentation of antigens to initiate a mucosal immune response (Brayden & Baird, 2004; Brayden et al., 2005). The adhesins required for attachment of pathogens to specific receptors on mucosal surfaces are being used for vaccine delivery via antigen-encapsulated microspheres (Wu et al., 2001; Byrd et al., 2005; Suzuki et al., 2006). Rotavirus selectively binds to rabbit M-cells via the viral haemagglutinin adhesin, α-1 protein. Administration of rotavirus protein α-1 conjugated with polylysine produces antigen-specific serum IgG and mucosal IgA responses (Kim et al., 2002). Biodegradable microparticles made from the copolymer poly-(dl-lactide-coglycide) (PLG) also show potential as mucosal delivery vehicles. PLG microspheres containing rotavirus antigen VP-6 DNA confer protection against challenge for up to 12 weeks. Microspheres coated with VP-6 are selectively taken up by sheep PP and induce VP-6-specific IgA following oral immunization of mice (Kim et al., 2002). PLG microparticles containing HIV peptides in combination with Ulex europaeus-1 lectin, which binds M-cell apical surfaces, generate both mucosal and systemic immune responses following intranasal immunization of mice (Manocha et al., 2005). A recent study has demonstrated efficient uptake of PEGylated PLGA-based nanoparticles displaying surface RGD molecules that successfully targeted β1 integrins on human M cells in cell culture and murine M cells in vivo (Garinot et al., 2007).

While particle uptake has been demonstrated in vivo in rodents and rabbits, it is unclear whether this is the case in humans. Human oral vaccine Phase 1 trials using E. coli enterotoxigenic E. coli (ETEC) antigens contained in biodegradable microspheres have been carried out but had poor outcomes and did not sufficiently stimulate mucosal immunity (Katz et al., 2003). Several pathogens target M-cells as a mode of entry into the host and are being genetically engineered to deliver antigen to mucosal inductive sites for induction of antigen-specific immune responses (Suzuki et al., 2006). Live-attenuated strains that have successfully been used as mucosal vaccines include the Sabin strain of poliovirus and Salmonella typhi Ty21a for polio and typhoid, respectively (Clark et al., 2001a, b). Investigation into the use of Salmonella species as live vectors for delivery of antigens and DNA is underway and one mutant lacking DNA adenine methylase has been shown previously to be unable to induce M-cell cytotoxicity but has an enhanced ability to promote an immune response (Clark et al., 2001a, b). Targeting ligand-coated particles to M-cells, combined with attempts to mimic pathogen entry routes via M-cells, could lead to increased uptake in vivo and successful initiation of mucosal immune responses.

Conclusion

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References

Despite relatively few numbers of M-cells within the intestinal epithelium compared with normal intestinal enterocytes, the nature of M-cells provides them with an important role in antigen sampling, bacterial translocation and initiation of mucosal immune responses. With the development of the in vitro M-cell model, research into these important immune cells has improved. However, the identification of specific M-cell antibodies and markers will significantly improve one's understanding of these cells. Furthermore, thorough research into the relationship between M-cells and the establishment of disease, and their ability to deliver antigen directly to mucosal immune initiation sites will improve delivery of existing mucosal vaccines and development of new strategies for oral vaccines.

References

  1. Top of page
  2. Abstract
  3. Introduction
  4. M-cells
  5. Origin and development of M-cells
  6. M-cell morphology
  7. M-cell function
  8. Entry sites for pathogens
  9. An in vitro M-cell model
  10. The future of M-cell research: vaccine delivery
  11. Conclusion
  12. References
  • Allan CH, Mendlick DL & Trier JS (1993) Rat intestinal M-cells contain acidic endosomal compartments and express class II major histocompatibility complex determination. Gastroenterology 24: 698708.
  • Amerongen HM, Weltzin RA, Format CM, Michetti P, Haseltine WA & Neutra MR (1991) Transepithelial transport of HIV-1 by intestinal M-cells: a mechanism for transmission of AIDS. J Acquir Immun Defic Syndr 4: 760765.
  • Amerongen HM, Wilson GA, Fields BN & Neutra MR (1994) Proteolytic processing of reovirus is required for adherence to intestinal M cells. J Virol 68: 84288432.
  • Autenrieth IB & Firsching R (1996) Penetration of M-cells and destruction of Peyer's patches by Yersinia enterocolitica: an ultrastructural and histological study. J Med Microbiol 44: 285294.
  • Baumler AJ, Tsolis RM & Heffron F (1996) The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches. Proc Natl Acad Sci USA 93: 279283.
  • Blanco LP & DiRita VJ (2006a) Bacterial-associated cholera toxin and GM1 binding are required for transcytosis of classical biotype Vibrio cholerae through an in vitro M-cell model system. Cell Microbiol 8: 982998.
  • Blanco LP & DiRita VJ (2006b) Antibodies enhance interaction of Vibrio cholerae with intestinal M-like cells. Infect Immun 74: 69576964.
  • Brayden DJ & Baird AW (2004) Apical membrane receptors on intestinal M-cells: potential targets for vaccine delivery. Adv Drug Deliv Rev 56: 721726.
  • Brayden DJ, Jepson MA & Baird AW (2005) Intestinal Peyer's patch M-cells and oral vaccine targeting. Drug Discov Today 10: 11451157.
  • Byrd W, DeLorimier A, Zheng Z & Cassels FJ (2005) Microencapsulated subunit vaccine approach to enterotoxigenic Escherichia coli and other mucosal pathogens. Adv Drug Deliv Rev 57: 13621380.
  • Cantey JR & Inman LR (1981) Diarrhea due to Escherichia coli strain RDEC-1 in the rabbit: Peyer's patch as the initial site of attachment and colonization. J Infect Dis 143: 440446.
  • Clark MA, Hirst BH & Jepson MA (1998a) M-cell surface beta-1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse Peyer's patch M-cells. Infect Immun 66: 12371243.
  • Clark MA, Hirst BH & Jepson MA (1998b) Inoculum composition and Salmonella pathogenicity island 1 regulate M-cell invasion and epithelial destruction by Salmonella typhimurium. Infect Immun 66: 724731.
  • Clark MA, Blair H, Liang L, Brey RN, Brayden D & Hirst BH (2001a) Targeting polymerised liposome vaccine carriers to intestinal M cells. Vaccine 20: 208217.
  • Clark MA, Jepson MA & Hirst BH (2001b) Exploiting M-cells for drug and vaccine delivery. Adv Drug Deliv Rev 50: 81106.
  • Corr SC, Hill C & Gahan CGM (2006) An in vitro cell-culture model demonstrates internalin- and hemolysin-independent translocation of Listeria monocytogenes across M-cells. Microb Pathog 41: 241250.
  • Daniels JD, Autenrieth IB & Goebel W (2000) Interaction of Listeria monocytogenes with the intestinal epithelium. FEMS Microbiol Lett 190: 323328.
  • Davis IC & Owen RL (1997) The immunopathology of M cells. Springer Semin Immunopathol 18: 421448.
  • DesRieux A, Ragnarsson EG, Gulberg E, Preat V, Schneider YJ & Artursson P (2005) Transport of nanoparticles across an in vitro model of the human intestinal follicle associated epithelium. Eur J Pharm Sci 25: 455465.
  • DesRieux A, Fievez V, Theate I, Mast J, Preat V & Schneider YJ (2007) An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M-cells. Eur J Pharm Sci 30: 380391.
  • Finzi G, Comaggi M, Capella C, Fiocca R, Basi F & Salcia E (1993) Cathepsin E in follicle-associated epithelium of intestine and tonsils: localization to M-cells and possible role in antigen processing histochemistry. Gut 99: 201211.
  • Forsberg A, Rosqvist R & Wolf-Watz H (1994) Regulation and polarized transfer of the Yersinia outer proteins (Yops) involved in antiphagocytosis. Trends Microbiol 2: 1419.
  • Fotopoulos G, Harari A, Michetti P, Trono D, Pantaleo G & Kraehenbuhl JP (2002) Transepithelial transport of HIV-1 by M-cells is receptor-mediated. Proc Natl Acad Sci USA 99: 94109414.
  • Garinot M, Fievez V, Pourcelle V et al. (2007) PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination. J Control Release 120: 195204.
  • Garvrieli Y, Sherman Y & Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labelling of nuclear DNA fragmentation. J Cell Biol 119: 493.
  • Gebert A & Hach G (1993) Differential binding of lectins to M-cells and enterocytes in the rabbit caecum. Gastroenterology 105: 13501361.
  • Gebert A, Rothkotter HJ & Pabst R (1996) M cells in Peyer's patches of the intestine. Int Rev Cytol 167: 91159.
  • Gebert A, Fassbender S, Werner K & Wiessferdt A (1999) The development of M cells in Peyer's patches is restricted to specialized dome-associated crypts. Am J Physiol 154: 15731582.
  • Ghosh S (2002) Intestinal entry of prions. Gastroenterology 40: 3739.
  • Giannasca PJ, Giannasca KT, Falk P, Gordon JI & Neutra MR (1994) Regional differences in glycoconjugates of intestinal M-cells in mice: potential targets for mucosal vaccines. Am J Physiol 267: 11081121.
  • Giannasca PJ, Giannasca KT, Leichtner AM & Neutra MR (1999) Human intestinal M-cells display the Sialyl Lewis A antigen. Infect Immun 67: 946953.
  • Goitsuka R, Chen CL, Benyon L, Asano Y, Kitamura D & Cooper MD (2007) Chicken cathelicidin-B1, an antimicrobial guardian at the mucosal M cell gateway. Proc Natl Acad Sci USA 104: 1506315068.
  • Grosdent N, Maridonneau-Parini I, Sory MP & Cornelis GR (2002) Role of Yops and adhesins in resistance of Yersinia enterocolitica to phagocytosis. Infect Immun 70: 41654176.
  • Hamon M, Bierne H & Cossart P (2006) Listeria monocytogenes: a multifaceted model. Nat Rev Microbiol 4: 423434.
  • Hamzaoui N, Kerneis S, Caliot E & Pringault E (2004) Expression and distribution of β1-integrins in in vitro-induced M-cells: implications for Yersinia adhesion to Peyer's patch epithelium. Cellular Microbiol 6: 817828.
  • Heath JP (1996) Epithelial cell migration in the intestine. Cell Biol Int 20: 139146.
  • Helander A, Silvey KJ, Mantis NJ, Hutchings AB, Chandran K, Lucas WT, Nibert ML & Neutra MR (2003) The viral sigma1 protein and glycoconjugates containing alpha2-3-linked sialic acid are involved in type 1 reovirus adherence to M cell apical surfaces. J Virol 77: 79647977.
  • Heppner FL, Christ AD, Klein MA, Prinz M, Fried M, Kraehenbuhl JP & Aguzzi A (2001) Transepithelial prion transport by M-cells. Nature 7: 976977.
  • Inman LR & Cantey JR (1983) Specific adherence of Escherichia coli (strain RDEC- 1) to membranous (M) cells of the Peyer's patch in Escherichia coli diarrhea in the rabbit. J Clin Invest 71: 18.
  • Jensen VB, Harty JT & Jones BD (1998) Interaction of the invasive pathogens Salmonella typhimurium, Listeria monocytogenes, and Shigella flexneri with M-cells and murine Peyer's patches. Infect Immun 66: 37583766.
  • Jepson MA, Clark MA, Foster N, Mason CM, Bennett MK, Simmons NL & Hirst BH (1996) Targeting to intestinal M-cells. J Anat 189: 507516.
  • Jones BD, Ghori N & Falkow S (1994) Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized epithelial M-cells of the Peyer's patches. J Exp Med 180: 1523.
  • Kanaya T, Aso H, Miyazawa K et al. (2007) Staining patterns for actin and villin distinguish M-cells in bovine follicle-associated epithelium. Res Vet Sci 82: 141149.
  • Katz DE, DeLorimier AJ, Wolf MK, Hall ER, Cassels FJ, VanHamont JE, Newcomer RL, Davachi MA, Taylor DN & McQueen CE (2003) Oral immunization of adult volunteers with microencapsulated enterotoxigenic Escherichia coli (ETEC) CS6 antigen. Vaccine 21: 341346.
  • Kerneis S, Bogdonova A, Kraehenbuhl JP & Pringault E (1997) Conversion by Peyer's patch lymphocytes of human enterocytes into M-cells that transport bacteria. Science 277: 949952.
  • Kim B, Bowersock T, Griebel P, Kidane A, Babiuk LA, Sanchez M, Attah-Poku S, Kaushik RS & Mutwiri GK (2002) Mucosal immune responses following oral immunization with rotavirus antigens encapsulated in alginate microspheres. J Contr Rel 85: 191202.
  • Kraehenbuhl JP & Neutra MR (1992) Molecular and cellular basis of immune protection of mucosal surfaces. Physiol Rev 72: 853879.
  • Lecuit M, Dramsi S, Gottardi C, Fedor-Chaiken M, Gumbiner B & Cossart P (1999) A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes. EMBO J 18: 3956963.
  • Liang E, Kabcenell AK, Coleman JR, Robson J, Ruffles R & Yazdanian M (2001) Permeability measurement of macromolecules and assessment of mucosal antigen sampling using in vitro converted M cells. J Pharmacol Toxicol Methods 46: 93101.
  • Manocha M, Pal PC, Chitralekha KT, Thomas BE, Tripathi V, Gupta SD, Paranjape R, Kulkarni S & Rao DN (2005) Enhanced mucosal and systemic immune response with intranasal immunization of mice with HIV peptides entrapped in PLG microparticles in comniation with Ulex Europaeus-1 lectin as M-cell target. Vaccine 23: 55995617.
  • Marchetti M, Sirard JC, Sansonetti PJ, Pringault E & Kerneis S (2004) Interaction of pathogenic bacteria with rabbit appendix M-cells: bacterial motility is a key feature in vivo. Microbes Infect 6: 521528.
  • Marco AJ, Altimira J, Prats N, Lopez S, Dominguez L, Domingo M & Briones V (1997) Penetration of Listeria monocytogenes in mice infected by the oral route. Microb Pathog 23: 255263.
  • Marra A & Isberg RR (1997) Invasin-dependent and invasion-independent pathways for translocation of Yersinia pseudotuberculosis across the Peyer's patch intestinal epithelium. Infect Immun 65: 34123421.
  • Martinez-Argudo I, Sands C & Jepson MA (2007) Translocation of enteropathogenic Escherichia coli across an in vitro M-cell model is regulated by its type III secretion system. Cellular Microbiol 9: 15381546.
  • McKee ML, Melton-Celsa AR, Moxley RA, Francis DH & O'Brien AD (1995) Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. Infect Immun 63: 37393744.
  • Neutra MR, Phillips TL, Mayer EL & Fishkind DJ (1987) Transport of membrane bound macromolecules by M-cells in follicle-associated epithelium of rabbit Peyer's patch. Cell Tissue Res 247: 537546.
  • Neutra MR, Frey A & Kraehenbuhl JP (1996a) Epithelial M-cells: gateways for mucosal infection and immunization. Cell 86: 345348.
  • Neutra MR, Pringault E & Kraehenbuhl JP (1996b) Antigen sampling across epithelial barriers and induction of mucosal immune responses. Annu Rev Immunol 14: 275300.
  • Neutra MR, Mantis NJ, Frey A & Giannasca PJ (1999) The composition and function of M-cell apical membranes: implications for microbial pathogenesis. Semin Immunol 11: 171181.
  • Neutra MR, Mantis NJ & Kraehenbuhl JP (2001) Collaboration of epithelial cells with organised mucosal mynphoid tissues. Nature Immunol 2: 10041009.
  • Nicoletti C (2000) Unsolved mysteries of intestinal M-cells. Gut 47: 735739.
  • Ouzilou L, Caliot E, Pelletier I, Prevost MC, Pringault E & Colbere-Garapin F (2002) Poliovirus transcytosis through M-like cells. J Gen Virol 83: 21772182.
  • Owen RL (1977) Sequential uptake of HRP by lymphoid follicle epithelium of Peyer's patches in normal unobstructed mouse intestine: an ultrastructural study. Gastroenterology 72: 440451.
  • Owen RL & Jones AL (1974) Epithelial cell specialization within human Peyer's patches: an ultrastructural study of intestinal lymphoid follicles. Gastroenterology 66: 189203.
  • Pappo J & Mahlman RT (1993) Follicle epithelial M-cells are a source of interleukin- 1 in Peyer's patches. Immunology 78: 505507.
  • Pentecost M, Otto G, Theriot JA & Amieva MR (2006) Listeria monocytogenes invades the epithelial junctions at sites of cell extrusion. PLos Pathog 2: e3.
  • Perdomo OJ, Cavaillon JM, Heurre M, Ohayon H, Gounon P & Sansonetti PJ (1994) Acute inflammation causes epithelial invasion and mucosal destruction in experimental shigellosis. J Exp Med 180: 13071319.
  • Phalipon A & Sansonetti PJ (2007) Shigella's ways of manipulating the host intestinal innate and adaptive immune system: a tool box for survival? Immunol Cell Biol 85: 119129.
  • Pielage JF, Cichon C, Greune L, Hirashima M, Kucharzik T & Schmidt MA (2007) Reversible differentiation of Caco-2 cells reveals galectin-9 as a surface marker molecule for human follicle-associated epithelia and M cell-like cells. Int J Biochem Cell Biol 39: 18861901.
  • Pierce NF, Kaper JB, Mekalanos JJ, Cray WC & Richardson K (1987) Determinants of the immunogenicity of live virulent and mutant Vibrio cholerae O1 in rabbit intestine. Infect Immun 55: 477481.
  • Pron B, Boumaila C, Jaubert F, Sarnacki S, Monnet JP, Berche P & Gaillard JL (1998) Comprehensive study of the intestinal stage of Listeriosis in a rat ligated loop system. Infect Immun 66: 747755.
  • Sansonetti PJ & Phalipon A (1999) M-cells as ports of entry for enteroinvasive pathogens: mechanisms of interaction, consequences for the disease process. Semin Immunol 11: 193203.
  • Sansonetti PJ, Arondel J, Cantey JR, Prevost MC & Heurre M (1996) Infection of rabbit Peyer's patches by Shigella flexneri: effect of adhesive or invasive bacterial phenotyes on follicle-associated epithelium. Infect Immun 64: 27522764.
  • Schulte R, Kerneis S, Klinke S, Bartels H, Preger S, Kraehenbuhl JP, Pringault E & Autenrieth IB (2000) Translocation of Yersinia enterocolitca across reconstituted intestinal epithelial monolayers is triggered by Yersinia invasion binding to β1 integrins apically expressed on M-cells. Cell Microbiol 2: 173185.
  • Sicinski P, Rowinski J, Warchol JB, Jarzabek Z, Gut W, Szczygiel B, Bielecki K & Kock G (1990) Poliovirus type-1 enters the human host through intestinal M-cells. Gastroenterology 98: 5658.
  • Sierro F, Pringault E, Assman PS, Kraehenbuhl JP & Debard N (2000) Transient expression of M-cell phenotype by enterocyte-like cells of the follicle-associated epithelium of mouse Peyer's patches. Gastroenterology 119: 734743.
  • Silvey KJ, Hutchings AB, Vajdy M, Petzke MM & Neutra MR (2001) Role of immunoglobulin A in protection against reovirus entry into Murine Peyer's patches. J Virol 75: 1087010879.
  • Suzuki T, Yoshikawa Y, Ashida H, Iwai H, Toyotome H, Matsui H & Sasakawa C (2006) High vaccine efficacy against shigellosis of recombinant non-invasive Shigella mutant that expresses Yersinia invasin. J Immunol 177: 470917.
  • Trier JS (1991) Structure and function of intestinal M-cells. Gastroenterol Clin North Am 20: 531547.
  • Tyrer P, Foxwell RA, Kyd J, Harvey M, Sizer P & Cripps A (2002) Validation and quantitation of an in vitro M-cell model. Biochem Biophys Res Commun 299: 377383.
  • Tyrer PC, Foxwell RA, Kyd J, Otczyk DC & Cripps A (2007) Receptor mediated targeting of M-cells. Vaccine 25: 32043209.
  • Vázquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Dominguez-Bernal G, Goebel W, Gonzalez-Zorn B, Wehland J & Kreft J (2001) Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 14: 584640.
  • Wu Y, Wang X, Csencsits KL, Haddad A, Walters N & Pascual DW (2001) M-cell targeted DNA vaccination. Proc Natl Acad Sci USA 98: 93189323.