Editor: Artur Ulmer
Influence of aging on murine neutrophil and macrophage function against Candida albicans
Article first published online: 28 JUN 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Volume 53, Issue 2, pages 214–221, July 2008
How to Cite
Murciano, C., Yáñez, A., O'Connor, J. E., Gozalbo, D. and Gil, M. L. (2008), Influence of aging on murine neutrophil and macrophage function against Candida albicans. FEMS Immunology & Medical Microbiology, 53: 214–221. doi: 10.1111/j.1574-695X.2008.00418.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received 13 February 2008; revised 12 March 2008; accepted 18 March 2008.First published online 28 April 2008.
- Candida albicans;
- innate immunity;
- murine macrophages and neutrophils;
Previous work by our group showed that aged C57BL/6 mice develop an altered innate and adaptive immune response to Candida albicans and are more susceptible to systemic primary candidiasis. In this work, we used young (2–3 months old) and aged (18–20 months old) C57BL/6 mice to study in vitro the influence of aging on (1) the fungicidal activity of neutrophils and macrophages, (2) the production of cytokines by resident peritoneal macrophages in response to C. albicans, and (3) cell surface Toll-like receptor (TLR) 2 expression on resident peritoneal macrophages. Our results indicate that murine phagocytes have a fungicidal activity well preserved with aging. In vitro production of proinflammatory cytokines (IL-6, IL-1β, and tumor necrosis factor-α and chemokines (MIP-2) by purified (CD11b+) peritoneal macrophages in response to yeasts and hyphae of C. albicans was significantly lower in aged mice as compared with young mice. However, the production of IL-10 by macrophages, in response to C. albicans, was similar in both young and aged animals. Moreover, baseline TLR2 surface expression level was lower on aged macrophages than on control macrophages. Taken together, these data indicate that the increased susceptibility to C. albicans disseminated infections in aged mice is correlated with defects in TLR2 expression and in cytokine production, but not with an impaired fungicidal activity.