Editor: Patrick Brennan
Cytokine release and expression induced by OmpA proteins from the Gram-negative anaerobes, Porphyromonas asaccharolytica and Bacteroides fragilis
Version of Record online: 28 JUN 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Volume 53, Issue 2, pages 252–259, July 2008
How to Cite
Magalashvili, L., Lazarovich, S., Pechatnikov, I., Wexler, H. M. and Nitzan, Y. (2008), Cytokine release and expression induced by OmpA proteins from the Gram-negative anaerobes, Porphyromonas asaccharolytica and Bacteroides fragilis. FEMS Immunology & Medical Microbiology, 53: 252–259. doi: 10.1111/j.1574-695X.2008.00423.x
- Issue online: 28 JUN 2008
- Version of Record online: 28 JUN 2008
- Received 4 December 2007; revised 7 April 2008; accepted 9 April 2008.First published online 23 May 2008.
- Porphyromonas asaccharolytica;
- Bacteroides fragilis;
OmpA proteins from Gram-negative anaerobes Porphyromonas asaccharolytica and Bacteroides fragilis induced release and expression of IL-1α, tumor necrosis factor (TNF)-α, IFN-γ, IL-6, and IL-10 from murine splenocytes in vitro in a dose-dependent fashion. The release of the cytokines induced by B. fragilis Bf-OmpA was at much lower levels compared with P. asaccharolytica Omp-PA; Bf-OmpA did not induce release of IL-10. Omp-PA and Bf-OmpA were able to upregulate mRNA expression of the tested cytokines. The results obtained with refolded Bf-OmpA were similar to those with native Bf-OmpA. The data presented in this research demonstrate for the first time that Omps from anaerobic bacteria can induce the release of cytokines, suggesting that Omp-PA and Bf-OmpA may play important roles in the pathogenic processes of these bacteria.