Editor: Patrick Brennan
cAMP levels within Mycobacterium tuberculosis and Mycobacterium bovis BCG increase upon infection of macrophages
Article first published online: 9 DEC 2008
© 2009 Wadsworth Center, New York State Department of Health. Journal compilation © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd
FEMS Immunology & Medical Microbiology
Volume 55, Issue 1, pages 68–73, January 2009
How to Cite
Bai, G., Schaak, D. D. and McDonough, K. A. (2009), cAMP levels within Mycobacterium tuberculosis and Mycobacterium bovis BCG increase upon infection of macrophages. FEMS Immunology & Medical Microbiology, 55: 68–73. doi: 10.1111/j.1574-695X.2008.00500.x
- Issue published online: 6 JAN 2009
- Article first published online: 9 DEC 2008
- Received 21 May 2008; revised 26 September 2008; accepted 23 October 2008.First published online 8 December 2008.
- Mycobacterium tuberculosis;
- Mycobacterium bovis BCG;
Adenosine 3′,5′-cyclic monophosphate (cAMP)-mediated signal transduction is common in both prokaryotes and eukaryotes, and several bacterial pathogens modulate cAMP signaling pathways of their mammalian hosts during infection. In this study, cAMP levels associated with Mycobacterium tuberculosis and Mycobacterium bovis BCG were measured during macrophage infection. cAMP levels within both bacteria increased c. 50-fold during infection of J774.16 macrophages, relative to the cAMP levels within bacteria incubated in tissue culture media alone. cAMP levels also increased within the macrophage cytoplasm upon uptake of live, but not dead, mycobacteria. The presence of albumin in the absence of oleic acid significantly decreased cAMP secretion and production by both M. tuberculosis and M. bovis BCG. These results suggest that cAMP signaling plays a role in the interaction of tuberculosis-complex mycobacteria with macrophages during infection, and that albumin may be a physiological indicator differentiating host environments during infection.