Editor: Svend Birkelund
Identification of dendritic cell subsets responding to genital infection by Chlamydia muridarum
Article first published online: 14 JAN 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Special Issue: Chlamydia
Volume 55, Issue 2, pages 226–236, March 2009
How to Cite
Moniz, R. J., Chan, A. M. and Kelly, K. A. (2009), Identification of dendritic cell subsets responding to genital infection by Chlamydia muridarum. FEMS Immunology & Medical Microbiology, 55: 226–236. doi: 10.1111/j.1574-695X.2008.00523.x
- Issue published online: 6 FEB 2009
- Article first published online: 14 JAN 2009
- Received 18 August 2008; revised 27 November 2008; accepted 9 December 2008.First published online 14 January 2009.
- Chlamydia muridarum;
- conventional dendritic cell;
- plasmacytoid dendritic cell
Dendritic cells (DCs) are central for the induction of T-cell responses needed for chlamydial eradication. Here, we report the activation of two DC subsets: a classical CD11b+ (cDC) and plasmacytoid (pDC) during genital infection with Chlamydia muridarum. Genital infection induced an influx of cDC and pDC into the genital tract and its draining lymph node (iliac lymph nodes, ILN) as well as colocalization with T cells in the ILN. Genital infection with C. muridarum also stimulated high levels of costimulatory molecules on cDC central for the activation of naïve T cells in vivo. In contrast, pDC expressed low levels of most costimulatory molecules in vivo and did not secrete cytokines associated with the production of T helper (Th)1 cells in vitro. However, pDC upregulated inducible costimulatory ligand expression and produced IL-6 and IL-10 in response to chlamydial exposure in vitro. Our findings show that these two DC subsets likely have different functions in vivo. cDCs are prepared for induction of antichlamydial T-cell responses, whereas pDCs have characteristics associated with the differentiation of non-Th1 cell subsets.