Editor: Gunna Christiansen
Determination of chlamydial load and immune parameters in asymptomatic, symptomatic and infertile women
Article first published online: 14 JAN 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Special Issue: Chlamydia
Volume 55, Issue 2, pages 250–257, March 2009
How to Cite
Agrawal, T., Vats, V., Salhan, S. and Mittal, A. (2009), Determination of chlamydial load and immune parameters in asymptomatic, symptomatic and infertile women. FEMS Immunology & Medical Microbiology, 55: 250–257. doi: 10.1111/j.1574-695X.2008.00530.x
- Issue published online: 6 FEB 2009
- Article first published online: 14 JAN 2009
- Received 17 August 2008; revised 13 December 2008; accepted 15 December 2008.First published online 14 January 2009.
- Chlamydia trachomatis;
- infectious load;
- immune response
The regulation of immune response and chlamydial infectious load in the cervix of human females is largely unknown. Infectious load in terms of inclusion-forming units (IFUs) was determined by quantitative cultures in Chlamydia-positive women, in asymptomatic women, women with mucopurulent cervicitis (MPC) and women with fertility disorders (FD). CD4+, CD8+, CD14+ cells, myeloid and plasmacytoid dendritic cells (mDCs and pDCs) in the cervix were quantified by flow cytometry. Cervical cytokines, levels of β-estradiol and C-reactive protein (CRP) in serum and cervical immunoglobulin A antibody to chlamydial major outer membrane protein antigen, chlamydial heat shock protein 60 and 10 antigens were measured by an enzyme-linked immunosorbent assay. In asymptomatic women, chlamydial load showed significant positive correlations with CD4, mDCs, interleukin-12 (IL-12) and IL-2; however, negative correlations were found with CD8 and IL-8 levels. In women with MPC, chlamydial IFUs correlated positively with CD8, pDC number, IL-8, CRP and interferon-γ (IFN-γ). In women with FD, chlamydial load showed a significant positive correlation with the pDC number, IL-10 and estradiol level and a negative correlation with CD4 and IFN-γ. Overall, these results suggest that the interplay between chlamydial infectious load and host immune responses may be the deciding factor for the clinical condition presented during Chlamydia trachomatis infection.