Editor: John Costerton
Biofilms in chronic bacterial prostatitis (NIH-II) and in prostatic calcifications
Article first published online: 17 FEB 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Special Issue: Biofilms
Volume 59, Issue 3, pages 337–344, August 2010
How to Cite
Mazzoli, S. (2010), Biofilms in chronic bacterial prostatitis (NIH-II) and in prostatic calcifications. FEMS Immunology & Medical Microbiology, 59: 337–344. doi: 10.1111/j.1574-695X.2010.00659.x
- Issue published online: 13 JUL 2010
- Article first published online: 17 FEB 2010
- Received 15 December 2009; accepted 5 February 2010.Final version published online 18 March 2010.
- Cat NIH-II chronic bacterial prostatitis;
- prostatic calcifications;
- prostate inflammation
The prevalence of inflammatory conditions of the prostate gland is increasing. In Italy, there is a high incidence of prostatitis (13.3%), also accompanied by prostatic calcifications. Cat NIH-II chronic bacterial prostatitis (CBPs) are the most frequent. Their aetiology theoretically involves the whole range of bacterial species that are able to form biofilms and infect prostate cells. The aim of our study was to isolate potential biofilm-producing bacteria from CBP patients, to evaluate their ability to produce in vitro biofilms, and to characterize intraprostatic bacteria and prostatic calcifications using scanning electron microscopy. The 150 clinical bacterial strains isolated from chronic prostatitis NIH-II patients were: 50 Enterococcus faecalis; 50 Staphylococcus spp.; 30 Escherichia coli; 20 gram-negative miscellanea. Quantitative assay of biofilm production and adhesion was performed according to the classic Christensen microwell assay. Isolates were classified as nonproducers, weak, moderate or strong producers. The majority of E. coli, gram-negative bacteria, Staphylococci and Enterococci strains were strong or medium producers: 63–30%, 75–15%, 46–36%, and 58–14%, respectively. Prostatic calcifications consisted of bacteria-like forms similar to the species isolated from biological materials and calcifications of patients. Our study proves, for the first time, that bacterial strains able to produce biofilms consistently are present in CBP. Additionally, prostatic calcifications are biofilm-related.