Editor: John Costerton
Transfer of antibiotic resistance by transformation with eDNA within oral biofilms
Article first published online: 17 FEB 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Special Issue: Biofilms
Volume 59, Issue 3, pages 345–349, August 2010
How to Cite
Hannan, S., Ready, D., Jasni, A. S., Rogers, M., Pratten, J. and Roberts, A. P. (2010), Transfer of antibiotic resistance by transformation with eDNA within oral biofilms. FEMS Immunology & Medical Microbiology, 59: 345–349. doi: 10.1111/j.1574-695X.2010.00661.x
Present addresses: Saad Hannan, Department of Neuroscience, Physiology, and Pharmacology, University College London, London, WC1E 6BT, UK. Michelle Rogers, 4.28 Royal School of Mines, Department of Bioengineering, Imperial College London, South Kensington, London, SW7 2AZ, UK.
- Issue published online: 13 JUL 2010
- Article first published online: 17 FEB 2010
- Received 8 December 2009; accepted 10 February 2010.Final version published online 10 March 2010.
- horizontal gene transfer;
We demonstrate that live donor Veillonella dispar cells can transfer the conjugative transposon Tn916 to four different Streptococcus spp. recipients in a multispecies oral consortium growing as a biofilm in a constant depth film fermentor. Additionally, we demonstrate that purified V. dispar DNA can transform Streptococcus mitis to tetracycline resistance in this experimental system. These data show that transfer of conjugative transposon-encoded antibiotic resistance can occur by transformation in addition to conjugation in biofilms.