Editor: Willem van Eden
γδ T cells promote the maturation of dendritic cells during West Nile virus infection
Article first published online: 17 FEB 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Volume 59, Issue 1, pages 71–80, June 2010
How to Cite
Fang, H., Welte, T., Zheng, X., Chang, G.-J. J., Holbrook, M. R., Soong, L. and Wang, T. (2010), γδ T cells promote the maturation of dendritic cells during West Nile virus infection. FEMS Immunology & Medical Microbiology, 59: 71–80. doi: 10.1111/j.1574-695X.2010.00663.x
- Issue published online: 4 MAY 2010
- Article first published online: 17 FEB 2010
- Received 28 October 2009; revised 4 February 2010; accepted 5 February 2010.Final version published online 10 March 2010.
- West Nile virus;
- dendritic cell;
- γδ T cell
γδ T cells are important for the early control of West Nile virus (WNV) dissemination. Here, we investigated the role of γδ T cells in the regulation of CD4+ T-cell response following a WNV challenge. Splenic dendritic cells (DCs) of WNV-infected γδ T-cell-deficient (TCRδ−/−) mice displayed lower levels of CD40, CD80, CD86 and major histocompatibility complex (MHC) class II expression and interleukin-12 (IL-12) production than those of wild-type mice. Naïve DCs cocultured with WNV-infected γδ T cells showed enhanced levels of costimulatory molecules, MHC class II expression and IL-12 production. Further, coculture of CD4+ T cells from OT II transgenic mice with DCs of WNV-infected TCRδ−/− mice induced less interferon-γ (IFN-γ) and IL-2 production than with those of wild-type controls. Viral antigens were detected in WNV-infected γδ T cells.WNV infection or toll-like receptor (TLR) agonist treatment of γδ T cells induced the production of IFN-γ, tumor necrosis factor-α and IL-6, which are known to promote DC maturation. Nevertheless, the levels of TLRs 2, 3, 4 and 7 expression of WNV-infected γδ T cells were not different from those of noninfected cells. Overall, these data suggest that WNV-induced γδ T-cell activation promotes DC maturation and initiates CD4+ T-cell priming.