Editor: Richard Marconi
Role of spx in biofilm formation of Staphylococcus epidermidis
Article first published online: 23 MAR 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Volume 59, Issue 2, pages 152–160, July 2010
How to Cite
Wang, C., Fan, J., Niu, C., Wang, C., Villaruz, A. E., Otto, M. and Gao, Q. (2010), Role of spx in biofilm formation of Staphylococcus epidermidis. FEMS Immunology & Medical Microbiology, 59: 152–160. doi: 10.1111/j.1574-695X.2010.00673.x
- Issue published online: 10 JUN 2010
- Article first published online: 23 MAR 2010
- Received 10 January 2010; revised 10 March 2010; accepted 14 March 2010.Final version published online 14 April 2010.
- Staphylococcus epidermidis;
- ClpP protease
Infections caused by the leading nosocomial pathogen Staphylococcus epidermidis are characterized by biofilm formation on implanted medical devices. In a previous study, we found that ClpP protease plays an essential role in biofilm formation of S. epidermidis. However, the mechanism by which ClpP impacts S. epidermidis biofilms has remained unknown. Here, we show that the Spx protein accumulates in the clpP mutant strain of S. epidermidis and controls biofilm formation of S. epidermidis via a pronounced effect on the transcription of the icaADBC operon coding for the production of the biofilm exopolysaccharide polysaccharide intercellular adhesion (PIA). Notably, in contrast to Staphylococcus aureus, Spx controls PIA expression via an icaR-independent mechanism. Furthermore, Spx affected primary surface attachment, although not by regulating the production of the autolysin AtlE. Our results indicate that ClpP enhances the formation of S. epidermidis biofilms by degrading Spx, a negative regulator of biofilm formation.