Editor: Mark Shirtliff
Characterization of MabA, a modulator of Lactobacillus rhamnosus GG adhesion and biofilm formation
Version of Record online: 9 APR 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Special Issue: Biofilms
Volume 59, Issue 3, pages 386–398, August 2010
How to Cite
Vélez, M. P., Petrova, M. I., Lebeer, S., Verhoeven, T. L.A., Claes, I., Lambrichts, I., Tynkkynen, S., Vanderleyden, J. and De Keersmaecker, S. C.J. (2010), Characterization of MabA, a modulator of Lactobacillus rhamnosus GG adhesion and biofilm formation. FEMS Immunology & Medical Microbiology, 59: 386–398. doi: 10.1111/j.1574-695X.2010.00680.x
- Issue online: 13 JUL 2010
- Version of Record online: 9 APR 2010
- Received 7 December 2009; revised 2 March 2010; accepted 31 March 2010.Final version published online 4 May 2010.
The probiotic Lactobacillus rhamnosus GG, first isolated from healthy human gut microbiota, has been reported to adhere very well to components of the intestinal mucosa, thereby enabling transient colonization of the gastrointestinal tract (GIT). In a search for the genes responsible for the good adherence capacity of this strain, a genomic region encoding a protein with homology to putative adhesion proteins (LGG_01865) and its putative regulator (LGG_01866) was identified. The sequence of the L. rhamnosus GG LGG_01865 encodes a polypeptide of 2419 amino acid residues containing 26 repetitive DUF1542 domains and a C-terminal LPxTG cell wall-anchoring motif. Phenotypic analyses of a dedicated LGG_01865 knockout mutant revealed a reduced biofilm formation capacity on abiotic surfaces and decreased adhesion to intestinal epithelial cells and tissues of the murine GIT. This suggests a modulating role for LGG_01865 in L. rhamnosus GG–host interactions. Therefore, we propose a new name for LGG_01865, i.e. MabA, modulator of adhesion and biofilm. Expression analysis indicated that LGG_01866 plays a conditional role in the regulation of LGG_01865 expression, i.e. when cells are grown under conditions of sugar starvation.