Editor: Gianfranco Donelli
An update on Pseudomonas aeruginosa biofilm formation, tolerance, and dispersal
Article first published online: 23 APR 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Special Issue: Biofilms
Volume 59, Issue 3, pages 253–268, August 2010
How to Cite
Harmsen, M., Yang, L., Pamp, S. J. and Tolker-Nielsen, T. (2010), An update on Pseudomonas aeruginosa biofilm formation, tolerance, and dispersal. FEMS Immunology & Medical Microbiology, 59: 253–268. doi: 10.1111/j.1574-695X.2010.00690.x
- Issue published online: 13 JUL 2010
- Article first published online: 23 APR 2010
- Received 5 January 2010; revised 16 March 2010; accepted 21 April 2010.Final version published online 18 May 2010.
- biofilm formation;
- Pseudomonas aeruginosa
We review the recent advances in the understanding of the Pseudomonas aeruginosa biofilm lifestyle from studies using in vitro laboratory setups such as flow chambers and microtiter trays. Recent work sheds light on the role of nutrients, motility, and quorum sensing in structure formation in P. aeruginosa biofilms. The second messenger, c-di-GMP, is established as an important regulator of the synthesis of polysaccharide and protein components of the biofilm matrix. Extracellular DNA is shown to be an essential component of the biofilm matrix. It has become apparent that biofilm formation involves interactions between different subpopulations. The molecular mechanisms underlying the tolerance of biofilm bacteria to antimicrobial agents are beginning to be unraveled, and new knowledge has been obtained regarding the environmental cues and regulatory mechanisms involved in biofilm dispersal.