Editor: Gianfranco Donelli
In vitro effect of branch extracts of Juniperus species from Turkey on Staphylococcus aureus biofilm
Version of Record online: 28 MAY 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Immunology & Medical Microbiology
Special Issue: Biofilms
Volume 59, Issue 3, pages 470–476, August 2010
How to Cite
Marino, A., Bellinghieri, V., Nostro, A., Miceli, N., Taviano, M. F., Güvenç, A. and Bisignano, G. (2010), In vitro effect of branch extracts of Juniperus species from Turkey on Staphylococcus aureus biofilm. FEMS Immunology & Medical Microbiology, 59: 470–476. doi: 10.1111/j.1574-695X.2010.00705.x
- Issue online: 13 JUL 2010
- Version of Record online: 28 MAY 2010
- Received 4 December 2009; revised 20 April 2010; accepted 14 May 2010.Final version published online 24 June 2010.
- Staphylococcus aureus;
- cell adhesion;
Methanol and aqueous branch extracts of five Juniperus species were examined for their effects on Staphylococcus aureus ATCC 6538P and S. aureus 810 biofilm. The Turkish plant material was Juniperus communis L. var. communis, J. communis L. var. saxatilis Pall., Juniperus drupacea Labill., Juniperus oxycedrus L. ssp. oxycedrus, J. oxycedrus L. ssp. macrocarpa (Sibth. & Sm.) Ball. The Juniperus extracts were subjected to preliminary phytochemical analysis by thin-layer chromatography. The antimicrobial activity was evaluated using the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The effects of the extracts on biofilm formation and preformed biofilm were quantified by both biomass OD and the CFU counting method. The phytochemical screening revealed the presence of polyphenols, coumarins, lignans, steroids, alkaloids and terpenes. For both strains, the MICs of all extracts were in the range of 4.88–78.12 μg mL−1. On S. aureus ATCC 6538P, the effects of subinhibitory concentration (0.5 MIC) of the extracts were minimal on planktonic growth and on adhering cells, whereas they were greater on biofilm formation. Differently, on S. aureus 810, they showed only a rather low efficacy on biofilm formation. The extracts at 2 MIC demonstrated a good activity on a preformed biofilm of S. aureus ATCC 6538P.