The lack of work dealing with possible ways of reducing biofilm production via inhibiting Candida albicans adherence in the first stage of biofilm formation was a motivation for this study. The study was focused on two questions: (1) can a decrease in adherence affect the quantity of mature biofilm? and (2) can blocking the surface C. albicans complement receptor 3-related protein (CR3-RP) with polyclonal anti-C3-RP antibody or monoclonal antibody OKM1 significantly contribute to a reduction in adherence during biofilm formation? The presence and quantity the CR3-RP expressed in the biofilm was confirmed by immunofluorescence, immunocytometry and enzyme-linked immunosorbent assay. To determine the changes in adherence of C. albicans CCY 29-3-162 and C. albicans catheter isolate, 30-, 60-, 90- and 120-min time points were selected and viability was determined by XTT assay. The strains were preincubated with both antibodies to block CR3-RP, which proved to be effective at reducing adhesion and the formation of a mature biofilm (64.1–74.6%). The duration of adhesion, between 30 and 120 min, seems to have a significant effect on the mature biofilm. The blocking of CR3-PR by antibodies before adherence affected the fitness of biofilm, which was not able to revitalize in the later stages.