Characterization of Staphylococcus aureus strains involved in human and bovine mastitis

  1. Susana Delgado1,2,
  2. Pilar García2,
  3. Leonides Fernández1,
  4. Esther Jiménez1,
  5. Mercedes Rodríguez-Baños3,
  6. Rosa del Campo3,
  7. Juan M. Rodríguez1

Article first published online: 9 MAY 2011

DOI: 10.1111/j.1574-695X.2011.00806.x

Issue

FEMS Immunology & Medical Microbiology

FEMS Immunology & Medical Microbiology

Volume 62, Issue 2, pages 225–235, July 2011

Additional Information

How to Cite

Delgado, S., García, P., Fernández, L., Jiménez, E., Rodríguez-Baños, M., del Campo, R. and Rodríguez, J. M. (2011), Characterization of Staphylococcus aureus strains involved in human and bovine mastitis. FEMS Immunology & Medical Microbiology, 62: 225–235. doi: 10.1111/j.1574-695X.2011.00806.x

Author Information

  1. 1

    Dpt. Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Madrid, Spain

  2. 2

    Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Asturias, Spain

  3. 3

    Servicio de Microbiología. Hospital Universitario Ramón y Cajal, Madrid, Spain

*Correspondence: Susana Delgado, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Carretera de Infiesto sn 33300 Villaviciosa, Asturias, Spain. Tel.: +34 985 892 131; fax: +34 989 892 233; e-mail: sdelgado@ipla.csic.es

Publication History

  1. Issue published online: 13 JUN 2011
  2. Article first published online: 9 MAY 2011
  3. Accepted manuscript online: 7 APR 2011 09:39AM EST
  4. Received 22 October 2010; revised 1 March 2011; accepted 27 March 2011., Final version published online 9 May 2011.

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Keywords:

  • Staphylococcus aureus ;
  • mastitis;
  • MLST;
  • genotyping;
  • virulence;
  • antibiotic resistance

Abstract

Staphylococcus aureus is one of the main etiological agents of mastitis in different mammalian species. At present, it is unknown whether strains isolated from human mastitis cases share phenotypic properties and genetic background with those obtained from animal mastitis cases. Therefore, the objective of this study was to characterize S. aureus strains isolated from women with lactational mastitis and to compare them with the strains responsible for bovine mastitis and noninfectious strains. All the strains were genotyped by both pulsed field gel electrophoresis and multilocus sequence typing and submitted to a characterization scheme that included diverse assays related to pathogenic potential and antibiotic resistance. Apart from siderophore production, no significant association was observed between the strains from bovine and human mastitis. Statistical differences between human- and bovine-mastitis-associated strains were detected for some traits and virulence determinants, such as the presence of prophages and cna and hlb genes, which were more frequently found within the bovine group. On the contrary, resistance to penicillin was significantly higher among strains isolated from human lactational mastitis, probably related to the common presence of the blaZ gene. A high genetic diversity was found among the strains involved in mastitis in breastfeeding women.

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