Effect of temperature and pH on the hopanoid content of Bacillus acidocaldarius

  1. Karl Poralla,
  2. Thomas Härtner,
  3. Elmar Kannenberg

Article first published online: 27 MAR 2006

DOI: 10.1111/j.1574-6968.1984.tb01073.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 23, Issue 2-3, pages 253–256, July 1984

Additional Information

How to Cite

Poralla, K., Härtner, T. and Kannenberg, E. (1984), Effect of temperature and pH on the hopanoid content of Bacillus acidocaldarius. FEMS Microbiology Letters, 23: 253–256. doi: 10.1111/j.1574-6968.1984.tb01073.x

Author Information

  1. Institut für Biologie II, Lehrbereich Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle 28, D-7400 Tübingen, F.R.G.

Publication History

  1. Issue published online: 27 MAR 2006
  2. Article first published online: 27 MAR 2006
  3. (Received 30 March 1984, Accepted 2 April 1984)

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Keywords:

  • Hopanoids;
  • temperature adaptation;
  • extreme environments;
  • Bacillus acidocaldarius

Abstract A gas-liquid chromatographic method for the quantitative determination of hopanoids with an elongated side chain was developed. After extraction of lipids from Bacillus acidocaldarius, periodate oxidation, reduction and acetylation the 1-hydroxyethane-29-hopane acetate was quantitated by comparison with 1-octadecanyl-glycerol ether. The contents of extended hopanoids increased strongly with increasing temperature and moderately with decreasing pH. Hopene showed no significant alteration with temperature and pH.

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