Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, U.K.
Maintenance coefficients and rates of turnover of cell material in Escherichia coli ML308 at different growth temperatures
Article first published online: 27 MAR 2006
FEMS Microbiology Letters
Volume 37, Issue 3, pages 317–320, December 1986
How to Cite
Wallace, R.John. and Holms, W.Harry. (1986), Maintenance coefficients and rates of turnover of cell material in Escherichia coli ML308 at different growth temperatures. FEMS Microbiology Letters, 37: 317–320. doi: 10.1111/j.1574-6968.1986.tb01816.x
- Issue published online: 27 MAR 2006
- Article first published online: 27 MAR 2006
- Received 27 August 1986, Accepted 28 August 1986
- Escherichia coli;
- maintenance coefficient;
Abstract Maintenance coefficients and rates of turnover of cell protein, cell walls and total cell phosphate were determined during the growth of Escherichia coli ML308 in fed-batch culture at different temperatures. The apparent Arrhenius activation energies (Ea) calculated for maintenance coefficients at temperatures over 30°C were 212–515 kJ· mol−1, indicative of a process thermodynamically similar to denaturation. The corresponding Arrhenius values for turnover were of a different magnitude, 44–115 kJ mol−1, more typical of enzyme-catalysed reactions. Addition of 0.5 M NaCl had a minor effect on maintenance at both temperatures. Turnover of cell material and salt homeostasis were therefore concluded to be minor components of the maintenance requirement of E. coli.