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Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis
Article first published online: 27 MAR 2006
DOI: 10.1111/j.1574-6968.1987.tb02071.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Earp, D. J., Ward, E.Sally. and Ellar, D. J. (1987), Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis. FEMS Microbiology Letters, 42: 195–199. doi: 10.1111/j.1574-6968.1987.tb02071.x
Publication History
- Issue published online: 27 MAR 2006
- Article first published online: 27 MAR 2006
- Received 28 January 1987, Accepted 17 February 1987
- Abstract
- References
- Cited By
Keywords:
- Bacillus thuringiensis;
- δ-Endotoxin gene;
- (DNA homology;
- Plasmid)
Abstract
The possible presence of protein δ-endotoxins with homology to a cloned mosquitocidal Bacillus thuringiensis var. israelensisδ-endotoxin was investigated in two other mosquitocidal B. thuringiensis strains. Antiserum raised against the var. israelensis 27-kDa toxic polypeptide cross-reacted strongly with a 27-kDa crystal polypeptide of var. PG14 (serotype 8a:8b, a serotype more usually associated only with lepidopteran toxicity). In addition hybridisation studies using a DNA probe derived from the cloned var. israelensis 27-kDa δ-endotoxin gene located a homologous region on a 72–74-MDa plasmid of PG14. A less toxic strain, var. Kyushuensis, showed neither immunological cross-reaction with the antiserum nor DNA hybridization with the probe.