Do heat shock proteins provide protection against freezing?
Article first published online: 27 MAR 2006
FEMS Microbiology Letters
Volume 72, Issue 1-2, pages 159–162, October 1990
How to Cite
Komatsu, Y., Kaul, S. C., Iwahashi, H. and Obuchi, K. (1990), Do heat shock proteins provide protection against freezing?. FEMS Microbiology Letters, 72: 159–162. doi: 10.1111/j.1574-6968.1990.tb03881.x
- Issue published online: 27 MAR 2006
- Article first published online: 27 MAR 2006
- (Received 19 March 1990, Revised 22 June 1990, Accepted 25 June 1990)
- Heat shock;
- Freezing protection;
- Hydrophobic hydration
Abstract Yeast cells were frozen by plunging directly into liquid nitrogen (LN2) after exposure at 43°C. Both the cells frozen without prior exposure to heat shock and those treated with cycloheximide showed almost 100% loss of viability during freezing and thawing. Heat exposure prior to freezing and thawing significantly increased the cell viability. This increase in cell viability was associated with the induction of heat shock protein synthesis, which was detected by gel electrophoresis. This protein may act by stabilizing the macromolecules and by increasing the hydrophobic interactions.