Isolation and characterization of staphyloferrin A, a compound with siderophore activity from Staphylococcus hyicus DSM 20459


Correspondence to: H. Zähner, Universität Tübingen, Biologie II, Mikrobiologie I, Auf der Morgenstelle 28, D-7400 Tübingen, F.R.G


A highly hydrophilic compound was isolated from low iron culture broth of Staphylococcus hyicus DSM 20459 which exhibits siderophore activity to the producer and 37 other staphylococci. The previously unknown metabolite was designated staphyloferrin A and constists of two molecules of citric acid, each linked to d-ornitine by an amide bond. Using an iron-pair HPLC-system we detected staphyloferrin A and a second iron regulated compound (staphyloferrin B) in the culture fluid of several Staphylococcus strains. We found no evidence that staphylococci synthesize catecholor hydroxamate-type siderophores.