Nucleotide sequence of the ethidium efflux gene from Escherichia coli


A.S. Purewal, Department of Veterinary Pathology, The Royal Veterinary College, Royal College Street, London NW1 0TU, U.K.


Abstract: The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11 960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium.