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Choline transport in Fusarium graminearum A 3 5

  1. G.D. Robson1,
  2. L.C. Best2,
  3. M.G. Wiebe1,
  4. A.P.J. Trinci1

Article first published online: 27 MAR 2006

DOI: 10.1111/j.1574-6968.1992.tb05269.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 92, Issue 3, pages 247–251, May 1992

Additional Information

How to Cite

Robson, G.D., Best, L.C., Wiebe, M.G. and Trinci, A.P.J. (1992), Choline transport in Fusarium graminearum A 3 5. FEMS Microbiology Letters, 92: 247–251. doi: 10.1111/j.1574-6968.1992.tb05269.x

Author Information

  1. 1

    Department of Cell and Structural Biology, Stopford Building, University of Manchester, Manchester, UK

  2. 2

    Department of Physiological Sciences and Medicine, School of Biological Sciences, Stopford Building, University of Manchester, Manchester, UK

Publication History

  1. Issue published online: 27 MAR 2006
  2. Article first published online: 27 MAR 2006
  3. (Received 15 January 1992, Accepted 14 February 1992)

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Keywords:

  • Choline transport;
  • Fungal morphology;
  • Fusarium graminearum

Abstract Fusarium graminearum A 35 possesses a high affinity system (Km/32 ± 8 μ M; mean ± SE) for uptake of choline, which was shown to be energy-dependent and constitutive. The maximum rate of choline uptake by this system was repressed by ammonia and glucose, showing a three-fold increase in maximum activity after nitrogen (2 h) or carbon (4 h) starvation. The system was highly specific for choline with only dimethylethanolamine (Ki= 198 ± 29 μ M), betaine aldehyde (Ki= 95 ± 14 μ M) and chlorocholine (Ki= 352 ± 40 μ M) acting as competitive inhibitors. Hemicholinium-3 acted as a mixed (non-competitive) inhibitor (KIES= 1.9 ± 0.6 μ M; KIE= 3.6 ± 1.9 μ M).

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