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Cloning of a Clostridium thermocellum DNA fragment encoding polypeptides that bind the catalytic components of the cellulosome
Article first published online: 27 MAR 2006
DOI: 10.1111/j.1574-6968.1992.tb05308.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Fujino, T., Béguin, P. and Aubert, J.-P. (1992), Cloning of a Clostridium thermocellum DNA fragment encoding polypeptides that bind the catalytic components of the cellulosome. FEMS Microbiology Letters, 94: 165–170. doi: 10.1111/j.1574-6968.1992.tb05308.x
Publication History
- Issue published online: 27 MAR 2006
- Article first published online: 27 MAR 2006
- (Received 13 April 1992, Accepted 23 April 1992)
- Abstract
- References
- Cited By
Keywords:
- Cellulase;
- Cellulosome;
- Scaffolding protein;
- Clostridium thermocellum
Abstract A test based on the binding of 125I-labelled endoglucanase CelD was used to clone a DNA region encoding at least two different polypeptides that interact with the conserved reiterated segment present in many catalytic components of the Clostridium thermocellum cellulosome. One of the polypeptides corresponds to the COOH-terminal region of the SL (or S1) component of the cellulosome (U.T. Gerngross and A.L. Demain, personal communication). It comprises repeated domains that are responsible for binding 125I-labelled CelD, and presumably represent anchoring sites for the various catalytic components of the cellulosome. The other polypeptide is encoded by a gene that has not yet been described.