Sequence and functional analysis of the cloned Neisseria meningitidis CMP-NeuNAc synthetase

  1. Ulrike Edwards,
  2. Matthias Frosch*

Article first published online: 27 MAR 2006

DOI: 10.1111/j.1574-6968.1992.tb05410.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 96, Issue 2-3, pages 161–166, September 1992

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How to Cite

Edwards, U. and Frosch, M. (1992), Sequence and functional analysis of the cloned Neisseria meningitidis CMP-NeuNAc synthetase. FEMS Microbiology Letters, 96: 161–166. doi: 10.1111/j.1574-6968.1992.tb05410.x

Author Information

  1. Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Hannover, FRG

* M. Frosch, Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, 3000 Hannover 61, FRG.

Publication History

  1. Issue published online: 27 MAR 2006
  2. Article first published online: 27 MAR 2006
  3. (Received 22 June 1992, Accepted 23 June 1992)

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Keywords:

  • Capsular polysaccharide biosynthesis;
  • N-Acetylneuraminic acid;
  • CMP-NeuNAc synthetase;
  • Neisseria meningitidis

Abstract The CMP-N-acetylneuraminic acid (CMP-NeuNAc) synthetase gene of Neisseria meningitidis group B is located on a 2.3-kb EcoRI fragment within the cps gene cluster. Nucleotide sequence determination of the gene encoding the CMP-NeuNAc synthetase revealed a 515-bp open reading frame that can encode a 18.9-kDA protein. A computer data base scan revealed a 59.4% identity to the CMP-NeuNAc synthetase gene of E. coli K1. Enzymatic activity was confirmed in vitro and in vivo. Transformation of the CMP-NeuNAc defective E. coli K1 strain EV5 with the meningococcal CMP-NeuNAc synthetase could complement the defect in E. coli.

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